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Literature summary for 3.1.26.5 extracted from
- Archaeal homologs of human RNase P protein pairs Pop5 with Rpp30 and Rpp21 with Rpp29 work on distinct functional domains of the RNA subunit (2010), Biosci. Biotechnol. Biochem., 74, 266-273.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| pET-15b-derived plasmid endcoding the C5 protein expressed in Escherichia coli BL21(DE3). Into pUC19 vector and transformed into Escherichia coli strain JM109 | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activation | Escherichia coli |  |
| Mg2+ | required for activation | Pyrococcus horikoshii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Pyrococcus horikoshii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | is unable to activate non-cognate RNase P RNAs, Pyrococcus horikoshii RNase P RNA and Escherichia coli RNase P RNA. Chimeric RNase P RNAs composed of the Escherichia coli RNA C-domain and Pyrococcus horikoshii S-domain or composed of the Pyrococcus horikoshii C-domain and Escherichia coli RNA S-domain, respectively, exhibit activity. C5 protein is involved in activation of the Escherichia coli pRNA C-domain | Escherichia coli | ? | - |
? | |
| additional information | RNase P proteins Pop5, Rpp21, Rpp29, Rpp30 and Rpp38 are unable to activate non-cognate RNase P RNAs, Pyrococcus horikoshii RNase P RNA and Escherichia coli RNase P RNA. Chimeric RNase P RNAs composed of the Escherichia coli RNA C-domain and Pyrococcus horikoshii S-domain or composed of the Pyrococcus horikoshii C-domain and Escherichia coli RNA S-domain, respectively, exhibit activity. Pop5 and Rpp30 are involved in activation of the Pyrococcus horikoshii pRNA C-domain, whereas Rpp21 and Rpp29 are implicated in stabilization of the Pyrococcus horikoshii pRNA S-domain | Pyrococcus horikoshii | ? | - |
? | |
| pre-tRNATyr + H2O | cleaves efficiently at a single phosphodieste bond between positions U59 and C60 | Escherichia coli | mature tRNATyr + 5'-oligoribonucleotide | - |
? | |
| pre-tRNATyr + H2O | is completely processed. Cleaves efficiently at a single phosphodieste bond between positions U59 and C60 | Pyrococcus horikoshii | mature tRNATyr + 5'-oligoribonucleotide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pop5 | - |
Pyrococcus horikoshii |
| Protein C5 | - |
Escherichia coli |
| ribonuclease P | - |
Escherichia coli |
| ribonuclease P | - |
Pyrococcus horikoshii |
| RNase P | - |
Escherichia coli |
| RNase P | - |
Pyrococcus horikoshii |
| Rpp21 | - |
Pyrococcus horikoshii |
| Rpp29 | - |
Pyrococcus horikoshii |
| Rpp30 | - |
Pyrococcus horikoshii |
| Rpp38 | - |
Pyrococcus horikoshii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Escherichia coli |
| 50 | - |
- |
Pyrococcus horikoshii |
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Release 2023.1 (January 2023)
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