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Literature summary for 3.1.26.5 extracted from

  • Paul, R.; Lazarev, D.; Altman, S.
    Characterization of RNase P from Thermotoga maritima (2001), Nucleic Acids Res., 29, 880-885.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the protein subunit in Escherichia coli Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000223
-
tRNATyr precursor pH 7.5, 50°C, enzyme RNA subunit Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
KCl activates, best at 200 mM Thermotoga maritima
Mg2+ optimal at 5-10 mM for the holoenzyme, the RNA subunit alone is active only at 100 mM MgCl2 Thermotoga maritima
NaCl activates, best at 50 mM Thermotoga maritima
NH4Cl activates, best at 200-400 mM Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein subunit from Escherichia coli, to homogeneity Thermotoga maritima

Renatured (Commentary)

Renatured (Comment) Organism
heterologous reconstitution of the holoenzyme using the RNA subunit from in vitro transcription and the recombinant protein subunit from overexpression in Escherichia coli, maximal activity at a ratio of 1:10 protein to RNA components Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNATyr precursor + H2O
-
Thermotoga maritima mature tRNATyr + 5'-terminal oligonucleotide
-
?

Synonyms

Synonyms Comment Organism
RNase P
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
optimal growth temperature of Thermotoga maritima is about 80°C Thermotoga maritima
50
-
about, recombinant holoenzyme Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
no activity at 65°C and above, recombinant holoenzyme Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
tRNATyr precursor pH 7.5, 50°C, enzyme RNA subunit Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
recombinant holoenzyme Thermotoga maritima