Cloned (Comment) | Organism |
---|---|
overexpression of the protein subunit in Escherichia coli | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000223 | - |
tRNATyr precursor | pH 7.5, 50°C, enzyme RNA subunit | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activates, best at 200 mM | Thermotoga maritima | |
Mg2+ | optimal at 5-10 mM for the holoenzyme, the RNA subunit alone is active only at 100 mM MgCl2 | Thermotoga maritima | |
NaCl | activates, best at 50 mM | Thermotoga maritima | |
NH4Cl | activates, best at 200-400 mM | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein subunit from Escherichia coli, to homogeneity | Thermotoga maritima |
Renatured (Comment) | Organism |
---|---|
heterologous reconstitution of the holoenzyme using the RNA subunit from in vitro transcription and the recombinant protein subunit from overexpression in Escherichia coli, maximal activity at a ratio of 1:10 protein to RNA components | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNATyr precursor + H2O | - |
Thermotoga maritima | mature tRNATyr + 5'-terminal oligonucleotide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase P | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
optimal growth temperature of Thermotoga maritima is about 80°C | Thermotoga maritima |
50 | - |
about, recombinant holoenzyme | Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
no activity at 65°C and above, recombinant holoenzyme | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
tRNATyr precursor | pH 7.5, 50°C, enzyme RNA subunit | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
recombinant holoenzyme | Thermotoga maritima |