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Literature summary for 3.1.26.5 extracted from
- RNase P: variations and uses (2002), J. Biol. Chem., 277, 6759-6762.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | specific external guide sequences offer a possibility for specific decrease of gene expression by inhibition of RNase P | Escherichia coli |
| pharmacology | specific external guide sequences offer a possibility for specific decrease of gene expression by inhibition of RNase P | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Cyanophora paradoxa | 9507 | - |
| cytoplasm | - |
Trypanosoma sp. | 5737 | - |
| cytoplasm | - |
Leishmania sp. | 5737 | - |
| mitochondrion | - |
Cyanophora paradoxa | 5739 | - |
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
| mitochondrion | - |
Aspergillus nidulans | 5739 | - |
| mitochondrion | - |
Trypanosoma sp. | 5739 | - |
| mitochondrion | - |
Leishmania sp. | 5739 | - |
| mitochondrion | the RNA subunits of the mitochondrial and nuclear enzymes are identical | Homo sapiens | 5739 | - |
| nucleus | - |
Cyanophora paradoxa | 5634 | - |
| nucleus | the RNA subunits of the mitochondrial and nuclear enzymes are identical | Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | divalent metal cations are essential | Cyanophora paradoxa | |
| additional information | divalent metal cations are essential | Bacillus subtilis | |
| additional information | divalent metal cations are essential | Mus musculus | |
| additional information | divalent metal cations are essential | Escherichia coli | |
| additional information | divalent metal cations are essential | Homo sapiens | |
| additional information | divalent metal cations are essential | Trypanosoma sp. | |
| additional information | divalent metal cations are essential | Leishmania sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
mitochondrial enzyme | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tRNA precursor + H2O | Cyanophora paradoxa | cleavage of 5'-terminal oligonucleotide, maturation, essential | mature tRNA + 5'-oligonucleotide | - |
? |  |
| tRNA precursor + H2O | Bacillus subtilis | cleavage of 5'-terminal oligonucleotide, maturation, essential | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | Mus musculus | cleavage of 5'-terminal oligonucleotide, maturation, essential | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | Escherichia coli | cleavage of 5'-terminal oligonucleotide, maturation, essential | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | Homo sapiens | cleavage of 5'-terminal oligonucleotide, maturation, essential | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | Saccharomyces cerevisiae | cleavage of 5'-terminal oligonucleotide, maturation, essential | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | Aspergillus nidulans | cleavage of 5'-terminal oligonucleotide, maturation, essential | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | Trypanosoma sp. | cleavage of 5'-terminal oligonucleotide, maturation, essential | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | Leishmania sp. | cleavage of 5'-terminal oligonucleotide, maturation, essential | mature tRNA + 5'-oligonucleotide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
- |
| Bacillus subtilis | - |
gene rnpB, type B enzyme | - |
| Cyanophora paradoxa | - |
- |
- |
| Escherichia coli | - |
gene rnpB, type A enzyme | - |
| Homo sapiens | - |
- |
- |
| Leishmania sp. | - |
- |
- |
| Mus musculus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Trypanosoma sp. | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| ribonucleoprotein | - |
Cyanophora paradoxa |
| ribonucleoprotein | - |
Mus musculus |
| ribonucleoprotein | - |
Homo sapiens |
| ribonucleoprotein | - |
Trypanosoma sp. |
| ribonucleoprotein | - |
Leishmania sp. |
| ribonucleoprotein | type A enzyme is a ribonucleoprotein and contains a single protein subunit | Escherichia coli |
| ribonucleoprotein | type B enzyme is a ribonucleoprotein and contains a single protein subunit | Bacillus subtilis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Aspergillus nidulans |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, the RNA subunit is essential for catalytic activity, divalent metal ion-dependent in-line SN2 displacement mechanism | Cyanophora paradoxa | |
| endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, the RNA subunit is essential for catalytic activity, divalent metal ion-dependent in-line SN2 displacement mechanism | Bacillus subtilis | |
| endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, the RNA subunit is essential for catalytic activity, divalent metal ion-dependent in-line SN2 displacement mechanism | Mus musculus | |
| endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, the RNA subunit is essential for catalytic activity, divalent metal ion-dependent in-line SN2 displacement mechanism | Escherichia coli | |
| endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, the RNA subunit is essential for catalytic activity, divalent metal ion-dependent in-line SN2 displacement mechanism | Homo sapiens | |
| endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, the RNA subunit is essential for catalytic activity, divalent metal ion-dependent in-line SN2 displacement mechanism | Leishmania sp. | |
| endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, the RNA subunit is essential for catalytic activity, except for the mitochondrial enzyme, divalent metal ion-dependent in-line SN2 displacement mechanism | Trypanosoma sp. |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reconstitution of a heterologous holoenzyme with a bacterial protein subunit and the plastid-encoded RNA subunit | Cyanophora paradoxa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| C-127 cell | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| influenza virus mRNA + H2O | - |
Homo sapiens | ? | - |
? | |
| influenza virus mRNA + H2O | cells transfected with virus | Mus musculus | ? | - |
? | |
| additional information | external guide sequences EGS can bind to complementary sequence of substrate ssRNA and thereby target the enzyme to specific cleavage sites | Mus musculus | ? | - |
? | |
| additional information | recognition of bipartite substrates and chimera constructed from external guide sequences EGS and ssRNA | Homo sapiens | ? | - |
? | |
| additional information | targeting of any mRNA for cleavage by the enzyme with aid of external guide sequences EGS, e.g. EGS with chloramphenicol acetyltransferase mRNA from cat, or EGS with gyrase A mRNA from Salmonella typhimurium, forming a complex with the complementary RNA substrate sequence, overview | Escherichia coli | ? | - |
? | |
| additional information | the RNA subunit alone is not active | Cyanophora paradoxa | ? | - |
? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation, essential | Cyanophora paradoxa | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation, essential | Bacillus subtilis | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation, essential | Mus musculus | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation, essential | Escherichia coli | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation, essential | Homo sapiens | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation, essential | Saccharomyces cerevisiae | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation, essential | Aspergillus nidulans | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation, essential | Trypanosoma sp. | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide, maturation, essential | Leishmania sp. | mature tRNA + 5'-oligonucleotide | - |
? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide | Cyanophora paradoxa | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide | Bacillus subtilis | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide | Mus musculus | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide | Escherichia coli | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide | Homo sapiens | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide | Saccharomyces cerevisiae | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide | Aspergillus nidulans | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide | Trypanosoma sp. | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? | |
| tRNA precursor + H2O | cleavage of 5'-terminal oligonucleotide | Leishmania sp. | mature tRNA + 5'-oligonucleotide | generates 5'-phosphate,3'-hydroxyl-product | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | ribozyme structure | Bacillus subtilis |
| More | ribozyme structure | Escherichia coli |
| More | the mitochondrial enzyme contains a single RNa subunit and a single protein subunit | Saccharomyces cerevisiae |
| More | the mitochondrial enzyme contains an RNA subunit and 7 protein subunits of 16-55 kDa | Aspergillus nidulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase P | - |
Cyanophora paradoxa |
| RNase P | - |
Bacillus subtilis |
| RNase P | - |
Mus musculus |
| RNase P | - |
Escherichia coli |
| RNase P | - |
Homo sapiens |
| RNase P | - |
Saccharomyces cerevisiae |
| RNase P | - |
Aspergillus nidulans |
| RNase P | - |
Trypanosoma sp. |
| RNase P | - |
Leishmania sp. |
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Release 2023.1 (January 2023)
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