Crystallization (Comment) | Organism |
---|---|
3 possible RNA-binding motifs, including a putative single-stranded RNA-binding cleft formed by an alpha-helix and a four-stranded beta-sheet, derived from crystal structure and NMR study | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | substitution of a sulfur atom for either the Rp or Sp nonbridging phosphate oxygen or the 3'oxyanion leaving group in pre-tRNA decreases the catalytic rate constant by over 1000fold | Bacillus subtilis | |
additional information | substitution of a sulfur atom for either the Rp or Sp nonbridging phosphate oxygen or the 3'oxyanion leaving group in pre-tRNA decreases the catalytic rate constant by over 1000fold | Escherichia coli | |
additional information | substitution of a sulfur atom for either the Rp or Sp nonbridging phosphate oxygen or the 3'oxyanion leaving group in pre-tRNA decreases the catalytic rate constant by over 1000fold | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | potential catalytic transition state structure including 3 required divalent metal ions, coordinated to nonbinding phosphate group oxygens | Bacillus subtilis | |
Mg2+ | potential catalytic transition state structure including 3 required divalent metal ions, coordinated to nonbinding phosphate group oxygens | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
ribonucleoprotein | - |
Staphylococcus aureus |
ribonucleoprotein | - |
Bacillus subtilis |
ribonucleoprotein | - |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules structure-function study, substrate recognition and catalytic mechanism of the ribozyme | Bacillus subtilis | |
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules structure-function study, substrate recognition and catalytic mechanism of the ribozyme | Escherichia coli | |
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor | an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, structure-function study, substrate recognition and catalytic mechanism of the ribozyme | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substitution of a sulfur atom for either the Rp or Sp nonbridging phosphate oxygen or the 3'oxyanion leaving group in pre-tRNA decreases the catalytic rate constant by over 1000fold | Staphylococcus aureus | ? | - |
? | |
additional information | substitution of a sulfur atom for either the Rp or Sp nonbridging phosphate oxygen or the 3'oxyanion leaving group in pre-tRNA decreases the catalytic rate constant by over 1000fold | Bacillus subtilis | ? | - |
? | |
additional information | substitution of a sulfur atom for either the Rp or Sp nonbridging phosphate oxygen or the 3'oxyanion leaving group in pre-tRNA decreases the catalytic rate constant by over 1000fold | Escherichia coli | ? | - |
? | |
tRNAAsp precursor + H2O | sequence | Staphylococcus aureus | mature tRNAAsp + 5'-terminal oligonucleotide | 5'-terminal oligonucleotide with 3'-hydroxyl and 5'-phosphoryl | ir | |
tRNAAsp precursor + H2O | sequence | Bacillus subtilis | mature tRNAAsp + 5'-terminal oligonucleotide | 5'-terminal oligonucleotide with 3'-hydroxyl and 5'-phosphoryl | ir | |
tRNAAsp precursor + H2O | sequence, potential catalytic transition state structure including 3 required divalent metal ions | Escherichia coli | mature tRNAAsp + 5'-terminal oligonucleotide | 5'-terminal oligonucleotide with 3'-hydroxyl and 5'-phosphoryl | ir |
Subunits | Comment | Organism |
---|---|---|
More | primary and secondary structure of the ribozymal RNA, catalytic domain and specificity domain, structure of the ribozyme plays an important role in catalysis, overview | Bacillus subtilis |
More | primary and secondary structure of the ribozymal RNA, catalytic domain and specificity domain, structure of the ribozyme plays an important role in catalysis, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
RNase P | - |
Staphylococcus aureus |
RNase P | - |
Bacillus subtilis |
RNase P | - |
Escherichia coli |