Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.5 extracted from

  • Lee, Y.C.; Lee, B.J.; Hwang, D.S.; Kang, H.S.
    Purification and characterization of mitochondrial ribonuclease P from Aspergillus nidulans (1996), Eur. J. Biochem., 235, 289-296.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
spermidine 2.5 mM increases activity by 2-4fold in presence of 5 mM MgCl2 Aspergillus nidulans

Inhibitors

Inhibitors Comment Organism Structure
K+ above 100 mM Aspergillus nidulans
Mg2+ above 40 mM Aspergillus nidulans
NH4+ above 100 mM Aspergillus nidulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00004
-
pre-tRNAAsp
-
Aspergillus nidulans

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Aspergillus nidulans 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ optimal concentration 40-60 mM Aspergillus nidulans
Mg2+ 10-15 mM required for optimal activity Aspergillus nidulans
NH4+ optimal concentration: 40-60 mM Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE Aspergillus nidulans
18000
-
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE Aspergillus nidulans
24000
-
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE Aspergillus nidulans
26000
-
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE Aspergillus nidulans
40000
-
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE Aspergillus nidulans
41000
-
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE Aspergillus nidulans
55000
-
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE Aspergillus nidulans
76000
-
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE Aspergillus nidulans
232000
-
glycerol density gradient sedimentation Aspergillus nidulans
440000
-
gel filtration Aspergillus nidulans

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
ribonucleoprotein cleaves mitochondrial precursor tRNAHis resulting in an 8-bp acceptor stem Aspergillus nidulans
ribonucleoprotein the enzyme requires an RNA as well as a protein subunit for its in vivo activity Aspergillus nidulans

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNA precursor + H2O cleaves synthetic pre-tRNAAsp by a single endonucleolytic action to generate 5'-end matured tRNA and an intact 5' leader Aspergillus nidulans tRNA + 5'-oligoribonucleotide
-
?
pre-tRNAAsp + H2O
-
Aspergillus nidulans mature tRNAAsp + 5'-oligonucleotide
-
?

Subunits

Subunits Comment Organism
octamer 1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE Aspergillus nidulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8.5
-
Aspergillus nidulans