Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermidine | 2.5 mM increases activity by 2-4fold in presence of 5 mM MgCl2 | Aspergillus nidulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K+ | above 100 mM | Aspergillus nidulans | |
Mg2+ | above 40 mM | Aspergillus nidulans | |
NH4+ | above 100 mM | Aspergillus nidulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
pre-tRNAAsp | - |
Aspergillus nidulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Aspergillus nidulans | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | optimal concentration 40-60 mM | Aspergillus nidulans | |
Mg2+ | 10-15 mM required for optimal activity | Aspergillus nidulans | |
NH4+ | optimal concentration: 40-60 mM | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE | Aspergillus nidulans |
18000 | - |
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE | Aspergillus nidulans |
24000 | - |
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE | Aspergillus nidulans |
26000 | - |
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE | Aspergillus nidulans |
40000 | - |
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE | Aspergillus nidulans |
41000 | - |
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE | Aspergillus nidulans |
55000 | - |
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE | Aspergillus nidulans |
76000 | - |
1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE | Aspergillus nidulans |
232000 | - |
glycerol density gradient sedimentation | Aspergillus nidulans |
440000 | - |
gel filtration | Aspergillus nidulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
ribonucleoprotein | cleaves mitochondrial precursor tRNAHis resulting in an 8-bp acceptor stem | Aspergillus nidulans |
ribonucleoprotein | the enzyme requires an RNA as well as a protein subunit for its in vivo activity | Aspergillus nidulans |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA precursor + H2O | cleaves synthetic pre-tRNAAsp by a single endonucleolytic action to generate 5'-end matured tRNA and an intact 5' leader | Aspergillus nidulans | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNAAsp + H2O | - |
Aspergillus nidulans | mature tRNAAsp + 5'-oligonucleotide | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 1 * 55000 + 1 * 41000, + 1 * 40000, + 1 * 26000, 1 * 24000, + 1 * 18000, + 1 * 16000, polypeptides, + 1 * 76000, RNA subunit, the enzyme is composed of seven polypeptides and an RNA moiety, the RNA component affects significantly the hydrodynamic properties of the RNase P enzyme, resulting in overestimation of the size of the ribonucleoprotein in gel filtration, SDS-PAGE | Aspergillus nidulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Aspergillus nidulans |