Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tRNA | - |
Escherichia coli | |
tRNA | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
additional information | not detectable in nucleoplasm | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Escherichia coli | |
K+ | monovalent cation required: Na+, K+ or NH4+ | Homo sapiens | |
Mg2+ | required | Escherichia coli | |
Mg2+ | required | Homo sapiens | |
Mg2+ | optimal activity at 5 mM | Homo sapiens | |
Na+ | monovalent cation required: Na+, K+ or NH4+ | Homo sapiens | |
NH4+ | - |
Escherichia coli | |
NH4+ | monovalent cation required: Na+, K+ or NH4+. Optimal NH4+ concentration is 200 mM | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA + H2O | Escherichia coli | - |
tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA + H2O | Homo sapiens | involved in biosynthesis of KB cell tRNA | tRNA + 5'-oligoribonucleotide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
KB cell | tissue culture | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA + H2O | - |
Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA + H2O | involved in biosynthesis of KB cell tRNA | Homo sapiens | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA precursor + H2O | single endonucleolytic scission in E. coli tRNATyr precursor, thereby separating the 41 extra nucleotides on the 5' end of the precursor molecule from the 5' terminal sequence of the mature tRNATyr molecule | Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA precursor + H2O | single endonucleolytic scission in E. coli tRNATyr precursor, thereby separating the 41 extra nucleotides on the 5' end of the precursor molecule from the 5' terminal sequence of the mature tRNATyr molecule | Homo sapiens | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA precursor + H2O | E. coli tRNATyr precursor | Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA precursor + H2O | E. coli tRNATyr precursor | Homo sapiens | tRNA + 5'-oligoribonucleotide | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |
8 | - |
broad optimum around pH 8.0 | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | less than 50% of maximal activity at pH 7.0 and 9.0 | Homo sapiens |