Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5-10 mM required | Bacillus subtilis | |
Mg2+ | 5-10 mM required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA + H2O | Bacillus subtilis | - |
tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA + H2O | Escherichia coli | - |
tRNA + 5'-oligoribonucleotide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
ribonucleoprotein | the RNA moiety can cleave tRNA precursor molecules in buffer containing either 60 mM Mg2+ or 10 mM Mg2+ plus 1 mM spermidine. Under these conditions the protein moiety of the enzymes alone shows no catalytic activity | Bacillus subtilis |
ribonucleoprotein | the RNA moiety can cleave tRNA precursor molecules in buffer containing either 60 mM Mg2+ or 10 mM Mg2+ plus 1 mM spermidine. Under these conditions the protein moiety of the enzymes alone shows no catalytic activity | Escherichia coli |
Purification (Comment) | Organism |
---|---|
RNA moiety | Bacillus subtilis |
RNA moiety | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4.5S RNA, precursor to + H2O | enzyme complex formed with M1 RNA from E. coli and the protein moiety from E. coli or Bacillus subtilis are active. No activity with the enzyme complex formed with M1 RNA from Bacillus subtilis and the protein from either bacterial species | Bacillus subtilis | ? | - |
? | |
4.5S RNA, precursor to + H2O | enzyme complex formed with M1 RNA from E. coli and the protein moiety from E. coli or Bacillus subtilis are active. No activity with the enzyme complex formed with M1 RNA from Bacillus subtilis and the protein from either bacterial species | Escherichia coli | ? | - |
? | |
pre-tRNA + H2O | - |
Bacillus subtilis | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA + H2O | - |
Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA precursor + H2O | RNA moiety can cleave pre-rRNA in buffers containing either 60 mM Mg2+ or 10 mM Mg2+ plus 1 mM spermidine | Bacillus subtilis | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA precursor + H2O | RNA moiety can cleave pre-rRNA in buffers containing either 60 mM Mg2+ or 10 mM Mg2+ plus 1 mM spermidine | Escherichia coli | tRNA + 5'-oligoribonucleotide | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | RNA and protein subunits from one species can complement subunits from the other species in reconstitution experiments | Bacillus subtilis |
More | RNA and protein subunits from one species can complement subunits from the other species in reconstitution experiments | Escherichia coli |