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Literature summary for 3.1.26.4 extracted from
- Phosphodiester cleavage in ribonuclease H occurs via an associative two-metal-aided catalytic mechanism (2008), J. Am. Chem. Soc., 130, 10955-10962.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D132N | perturbs the coordination shell of the A-site Mg2+ | Halalkalibacterium halodurans |
| D192N | crystallographic model. Although the mutant enzyme is completely inactive, D192N substitution supposedly does not significantly affect the active site architecture | Halalkalibacterium halodurans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the two Mg2+ support the formation of a meta-stable phosphorane intermediate along the reaction | Halalkalibacterium halodurans |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halalkalibacterium halodurans | Q9KEI9 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA-RNA hybrid + H2O | - |
Halalkalibacterium halodurans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease H | - |
Halalkalibacterium halodurans |
| RNase H | - |
Halalkalibacterium halodurans |
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Release 2023.1 (January 2023)
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