Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | ribonuclease H I acts optimally at about 30 mM (NH4)2SO4 in the case of Mn2+ activation, in the presence of Mg2+ ions the enzyme acts best if (NH4)2SO4 is omitted | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | the Mg2+-dependent activity is inhibited by 60% at 20 mM. The Mn2+-dependent activity is unaffected | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | ribonuclease H I | Bos taurus | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal activity at 10 mM | Bos taurus | |
Mn2+ | optimal activity at 1 mM | Bos taurus | |
additional information | the enzyme exists in two different conformations depending on the type of divalent cation activation | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24300 | - |
1 * 31600, A, + 2 * 26600, B, + 1 * 24800, C, + 1 * 24300, D, SDS-PAGE | Bos taurus |
24800 | - |
1 * 31600, A, + 2 * 26600, B, + 1 * 24800, C, + 1 * 24300, D, SDS-PAGE | Bos taurus |
26600 | - |
1 * 31600, A, + 2 * 26600, B, + 1 * 24800, C, + 1 * 24300, D, SDS-PAGE | Bos taurus |
31600 | - |
1 * 31600, A, + 2 * 26600, B, + 1 * 24800, C, + 1 * 24300, D, SDS-PAGE | Bos taurus |
80000 | - |
calculation from sedimentation behavior | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
large scale purification | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA hybrid + H2O | - |
Bos taurus | ribonucleotide 5'-phosphomonoester | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 1 * 31600, A, + 2 * 26600, B, + 1 * 24800, C, + 1 * 24300, D, SDS-PAGE | Bos taurus |