Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosylhomocysteine | - |
Bos taurus | |
S-adenosylmethionine | at 35°C but not at 0°C | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates cleavage of only poly(A) hybrids | Bos taurus | |
Mg2+ | activates cleavage of only the hybrid combinations containing purine ribo strands | Bos taurus | |
Mn2+ | activates enzymatic cleavage of all hybrid combinations | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA hybrid + H2O | DNA-RNA hybrid made from phage f1 DNA | Bos taurus | ribonucleotide 5'-phosphomonoester | the bulk of the resulting poly(dA) obtained by cleavage of poly(dT)*poly(A4)-(dA)x still retains one covalently linked riboadenylic acid end group, a small portion carries a ribo dinucleotide | ? | |
RNA-DNA hybrid + H2O | deoxyribotetranucleotides can still be cleaved | Bos taurus | ribonucleotide 5'-phosphomonoester | the bulk of the resulting poly(dA) obtained by cleavage of poly(dT)*poly(A4)-(dA)x still retains one covalently linked riboadenylic acid end group, a small portion carries a ribo dinucleotide | ? | |
RNA-DNA hybrid + H2O | poly(dT)'poly(A) in the presence of Mg2+ is the best substrate, poly(dC)'poly(G) is attacked much more slowly. Degradation velocity rises with the increasing length of the deoxyribo strand. The efficieny decreases in the following order: (dA)4-poly(U), (dG)4*poly(C), (dC)4*poly(G), (dT)4*poly(A) | Bos taurus | ribonucleotide 5'-phosphomonoester | the bulk of the resulting poly(dA) obtained by cleavage of poly(dT)*poly(A4)-(dA)x still retains one covalently linked riboadenylic acid end group, a small portion carries a ribo dinucleotide | ? |