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Literature summary for 3.1.26.4 extracted from

  • Stavrianopoulos, J.G.; Gambino-Giuffrida, A.; Chargraff, E.
    Ribonuclease H of calf thymus: substrate specificity, activation, inhibition (1976), Proc. Natl. Acad. Sci. USA, 73, 1087-1091.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
S-adenosylhomocysteine
-
Bos taurus
S-adenosylmethionine at 35°C but not at 0°C Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates cleavage of only poly(A) hybrids Bos taurus
Mg2+ activates cleavage of only the hybrid combinations containing purine ribo strands Bos taurus
Mn2+ activates enzymatic cleavage of all hybrid combinations Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA hybrid + H2O DNA-RNA hybrid made from phage f1 DNA Bos taurus ribonucleotide 5'-phosphomonoester the bulk of the resulting poly(dA) obtained by cleavage of poly(dT)*poly(A4)-(dA)x still retains one covalently linked riboadenylic acid end group, a small portion carries a ribo dinucleotide ?
RNA-DNA hybrid + H2O deoxyribotetranucleotides can still be cleaved Bos taurus ribonucleotide 5'-phosphomonoester the bulk of the resulting poly(dA) obtained by cleavage of poly(dT)*poly(A4)-(dA)x still retains one covalently linked riboadenylic acid end group, a small portion carries a ribo dinucleotide ?
RNA-DNA hybrid + H2O poly(dT)'poly(A) in the presence of Mg2+ is the best substrate, poly(dC)'poly(G) is attacked much more slowly. Degradation velocity rises with the increasing length of the deoxyribo strand. The efficieny decreases in the following order: (dA)4-poly(U), (dG)4*poly(C), (dC)4*poly(G), (dT)4*poly(A) Bos taurus ribonucleotide 5'-phosphomonoester the bulk of the resulting poly(dA) obtained by cleavage of poly(dT)*poly(A4)-(dA)x still retains one covalently linked riboadenylic acid end group, a small portion carries a ribo dinucleotide ?