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Literature summary for 3.1.26.13 extracted from

  • Wang, J.; Bambara, R.A.; Demeter, L.M.; Dykes, C.
    Reduced fitness in cell culture of HIV-1 with nonnucleoside reverse transcriptase inhibitor-resistant mutations correlates with relative levels of reverse transcriptase content and RNase H activity in virions (2010), J. Virol., 84, 9377-9389.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
G190A site-directed mutagenesis, the mutant virus shows moderately reduced fitness compared to that of the wild-type virus Human immunodeficiency virus 1
G190S site-directed mutagenesis, the mutant virus shows moderately reduced fitness compared to that of the wild-type virus Human immunodeficiency virus 1
K103N site-directed mutagenesis, the mutant virus shows fitness similar to that of the wild-type virus Human immunodeficiency virus 1
P236L site-directed mutagenesis, the mutant virus shows substantially reduced fitness compared to that of the wild-type virus Human immunodeficiency virus 1
V106A site-directed mutagenesis, the mutant virus shows moderately reduced fitness compared to that of the wild-type virus Human immunodeficiency virus 1
Y181C site-directed mutagenesis, the mutant virus shows fitness similar to that of the wild-type virus Human immunodeficiency virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information pre-steady-state kinetics, overview Human immunodeficiency virus 1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Human immunodeficiency virus 1 5'end-directed RNase H of reverse transcriptase ?
-
?

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
HIV-1
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information virus passage in human 293 cells Human immunodeficiency virus 1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 5'end-directed RNase H of reverse transcriptase Human immunodeficiency virus 1 ?
-
?

Synonyms

Synonyms Comment Organism
RNase H
-
Human immunodeficiency virus 1

General Information

General Information Comment Organism
malfunction severe defects in RNase H activity alone, exemplified by the P236L mutant, appear sufficient to cause a substantial reduction in fitness. Reductions in reverse transcriptase content decrease both polymerization and RNase H activity in virions Human immunodeficiency virus 1