Protein Variants | Comment | Organism |
---|---|---|
G190A | site-directed mutagenesis, the mutant virus shows moderately reduced fitness compared to that of the wild-type virus | Human immunodeficiency virus 1 |
G190S | site-directed mutagenesis, the mutant virus shows moderately reduced fitness compared to that of the wild-type virus | Human immunodeficiency virus 1 |
K103N | site-directed mutagenesis, the mutant virus shows fitness similar to that of the wild-type virus | Human immunodeficiency virus 1 |
P236L | site-directed mutagenesis, the mutant virus shows substantially reduced fitness compared to that of the wild-type virus | Human immunodeficiency virus 1 |
V106A | site-directed mutagenesis, the mutant virus shows moderately reduced fitness compared to that of the wild-type virus | Human immunodeficiency virus 1 |
Y181C | site-directed mutagenesis, the mutant virus shows fitness similar to that of the wild-type virus | Human immunodeficiency virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pre-steady-state kinetics, overview | Human immunodeficiency virus 1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Human immunodeficiency virus 1 | 5'end-directed RNase H of reverse transcriptase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
HIV-1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | virus passage in human 293 cells | Human immunodeficiency virus 1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 5'end-directed RNase H of reverse transcriptase | Human immunodeficiency virus 1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase H | - |
Human immunodeficiency virus 1 |
General Information | Comment | Organism |
---|---|---|
malfunction | severe defects in RNase H activity alone, exemplified by the P236L mutant, appear sufficient to cause a substantial reduction in fitness. Reductions in reverse transcriptase content decrease both polymerization and RNase H activity in virions | Human immunodeficiency virus 1 |