Cloned (Comment) | Organism |
---|---|
transfection of HEK 293 cells | Human immunodeficiency virus 1 |
Protein Variants | Comment | Organism |
---|---|---|
I505A | no discernible effect on viral titer | Human immunodeficiency virus 1 |
K476A | mutation reduces the viral titer less than 2fold | Human immunodeficiency virus 1 |
N474A | mutation reduces the viral titer less than 2fold | Human immunodeficiency virus 1 |
N474A/Q475A | mutation reduces the viral titer 5- to 10fold, , reduction in the efficiency of DNA synthesis. Mutant is less efficient than the wild-type enzyme in its ability to remove a polypurine tract primer from a model substrate and has an altered RNase H cleavage specificity | Human immunodeficiency virus 1 |
Q475A | mutation reduces the viral titer 5- to 10fold | Human immunodeficiency virus 1 |
Q500A | mutation reduces the viral titer less than 2-fold | Human immunodeficiency virus 1 |
R448A | no discernible effect on viral titer | Human immunodeficiency virus 1 |
T473A | mutation abolishes viral replication | Human immunodeficiency virus 1 |
Y501A | mutation reduces the viral titer 5- to 10fold, reduction in the efficiency of DNA synthesis. Mutant is less efficient than the wild-type enzyme in its ability to remove a polypurine tract primer from a model substrate and has an altered RNase H cleavage specificity | Human immunodeficiency virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | interdependence of the polymerase and RNase H activities of HIV-1 reverse transcription during viral DNA synthesis | Human immunodeficiency virus 1 | ? | - |
? |