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Literature summary for 3.1.26.13 extracted from

  • Brehm, J.H.; Mellors, J.W.; Sluis-Cremer, N.
    Mechanism by which a glutamine to leucine substitution at residue 509 in the ribonuclease H domain of HIV-1 reverse transcriptase confers zidovudine resistance (2008), Biochemistry, 47, 14020-14027.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.13

Activating Compound

Activating Compound Comment Organism Structure
ATP activates, effect on kinetics, overview Human immunodeficiency virus 1

Protein Variants

Protein Variants Comment Organism
D67N/K70R/T215F site-directed mutagenesis Human immunodeficiency virus 1
D67N/K70R/T215F/Q509L site-directed mutagenesis Human immunodeficiency virus 1
G509L Glu to Leu substitution at residue 509 in the ribonuclease H domain of HIV-1 reverse transcriptase confers zidovudine resistance, mechanism, overview. Q509L increases zidovudine monophosphate excision activity of RT on RNA/DNA template/primers, but not DNA/DNA template/primers, due to Q509L decreasing a secondary RNase H cleavage event that reduces the RNA/DNA duplex length to 10 nucleotides and significantly impairs the enzyme's ability to excise the chain-terminating nucleotide. Mutation Q509L does not affect initial rates of the polymerase-directed RNase H activity but only polymerase-independent cleavages that occur after a template/primer dissociation event. Q509L decreases the affinity of the enzyme to bind template/primers with duplex lengths less than 18 nucleotides in the polymerase-independent RNase H cleavage mode, while not affecting the enzyme's affinity to bind the same template/primers in an zidovudine monophosphate excision competent mode Human immunodeficiency virus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information presteady-state kinetic analyses, overview Human immunodeficiency virus 1

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Human immunodeficiency virus 1

Organism

Organism UniProt Comment Textmining
Human immunodeficiency virus 1
-
 HIV-1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a 5'-32P-labeled AZT-MP chain-terminated RNA/DNA template/primer substrate Human immunodeficiency virus 1 ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase H
-
 Human immunodeficiency virus 1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Human immunodeficiency virus 1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Human immunodeficiency virus 1