Cloned (Comment) | Organism |
---|---|
fragments of RNase E, either containing the enolase-binding microdomain (residues 833-851), the PNPase-binding site (residues 844-1061) or both (residues 734-1061) | Escherichia coli |
rne gene amplified and subcloned into pCR-BluntII-TOPO vector to generate pTOPOS14Rne. Expression plasmid pBADS14Rne constructed by excising the rne gene as a 3.3 kb gene Nco I-Pme I fragment from pTOPOS14Rne and inserting the fragment into the medium copy number plasmid pBADlacZ, putting RNase E expression under the control of the arabinose inducible promoter PBAD, and transformed into Escherichia coli AC23 (rne-1). RNase E putative enolase-binding microdomain (residues 885-909) and the putative PNPase-binding microdomain from S14 RNase E (residues 1015-1094) cloned | Photobacterium angustum S14 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
122600 | - |
calculated from sequence | Photobacterium angustum S14 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P21513 | - |
- |
Photobacterium angustum S14 | Q1ZS71 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
16s rRNA + H2O | - |
Escherichia coli | ? | - |
? | |
16s rRNA + H2O | RNase E completely suppresses the accumulation of the 16.5S RNA intermediate in the Escherichia coli rne-1 strain | Photobacterium angustum S14 | ? | - |
? | |
additional information | Escherichia coli enolase and its RNase E enolase-binding site demonstrate positive interactions. PNPase-binding site of RNase E interacts with Vibrio angustum S14 or Escherichia coli PNPase | Escherichia coli | ? | - |
? | |
additional information | RNase E enolase-binding site interacts with enolase from both Vibrio angustum S14 and Escherichia coli. The C-terminal half of RNase E interacts with Vibrio angustum S14 or Escherichia coli PNPase. C-terminal half of RNase E is capable of self-interaction | Photobacterium angustum S14 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase E | - |
Escherichia coli |
RNase E | - |
Photobacterium angustum S14 |
General Information | Comment | Organism |
---|---|---|
physiological function | RNase E microdomain sequences are well-conserved with those described in Escherichia coli. The RNase E C-terminal half is less conserved and structured than its N-terminal half. RNase E is a hub protein with multiple interaction interfaces that are under different evolutionary pressures. RNase E can rescue the temperature sensitive rne-1 phenotype of Escherichia coli | Photobacterium angustum S14 |