Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.12 extracted from

  • Erce, M.A.; Low, J.K.; March, P.E.; Wilkins, M.R.; Takayama, K.M.
    Identification and functional analysis of RNase E of Vibrio angustum S14 and two-hybrid analysis of its interaction partners (2009), Biochim. Biophys. Acta, 1794, 1107-1114.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
fragments of RNase E, either containing the enolase-binding microdomain (residues 833-851), the PNPase-binding site (residues 844-1061) or both (residues 734-1061) Escherichia coli
rne gene amplified and subcloned into pCR-BluntII-TOPO vector to generate pTOPOS14Rne. Expression plasmid pBADS14Rne constructed by excising the rne gene as a 3.3 kb gene Nco I-Pme I fragment from pTOPOS14Rne and inserting the fragment into the medium copy number plasmid pBADlacZ, putting RNase E expression under the control of the arabinose inducible promoter PBAD, and transformed into Escherichia coli AC23 (rne-1). RNase E putative enolase-binding microdomain (residues 885-909) and the putative PNPase-binding microdomain from S14 RNase E (residues 1015-1094) cloned Photobacterium angustum S14

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
122600
-
calculated from sequence Photobacterium angustum S14

Organism

Organism UniProt Comment Textmining
Escherichia coli P21513
-
-
Photobacterium angustum S14 Q1ZS71
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
16s rRNA + H2O
-
Escherichia coli ?
-
?
16s rRNA + H2O RNase E completely suppresses the accumulation of the 16.5S RNA intermediate in the Escherichia coli rne-1 strain Photobacterium angustum S14 ?
-
?
additional information Escherichia coli enolase and its RNase E enolase-binding site demonstrate positive interactions. PNPase-binding site of RNase E interacts with Vibrio angustum S14 or Escherichia coli PNPase Escherichia coli ?
-
?
additional information RNase E enolase-binding site interacts with enolase from both Vibrio angustum S14 and Escherichia coli. The C-terminal half of RNase E interacts with Vibrio angustum S14 or Escherichia coli PNPase. C-terminal half of RNase E is capable of self-interaction Photobacterium angustum S14 ?
-
?

Synonyms

Synonyms Comment Organism
RNase E
-
Escherichia coli
RNase E
-
Photobacterium angustum S14

General Information

General Information Comment Organism
physiological function RNase E microdomain sequences are well-conserved with those described in Escherichia coli. The RNase E C-terminal half is less conserved and structured than its N-terminal half. RNase E is a hub protein with multiple interaction interfaces that are under different evolutionary pressures. RNase E can rescue the temperature sensitive rne-1 phenotype of Escherichia coli Photobacterium angustum S14