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Literature summary for 3.1.26.11 extracted from

  • Minagawa, A.; Ishii, R.; Takaku, H.; Yokoyama, S.; Nashimoto, M.
    The flexible arm of tRNase Z is not essential for pre-tRNA binding but affects cleavage site selection (2008), J. Mol. Biol., 381, 289-299.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tag Bacillus subtilis
His-tag Escherichia coli
His-tag Thermoplasma acidophilum
His-tag Thermotoga maritima
His-tag Pyrobaculum aerophilum

Protein Variants

Protein Variants Comment Organism
K162L residue at the flexible arm of tRNase Z Thermotoga maritima
K162L/R166L/K169L residues at the flexible arm of tRNase Z Thermotoga maritima
K162R residue at the flexible arm of tRNase Z Thermotoga maritima
K169L residue at the flexible arm of tRNase Z Thermotoga maritima
K169R residue at the flexible arm of tRNase Z Thermotoga maritima
Q44S very low activity Pyrobaculum aerophilum
Q44S/Q46T double mutant, cleavage of human pre-tRNA(Arg) after U75, very inefficiently Escherichia coli
Q45S/Q47T very low activity Thermoplasma acidophilum
R166L residue at the flexible arm of tRNase Z Thermotoga maritima
S31Q cleavage site selection affected Thermotoga maritima
S31Q/T33Q cleavage site analyzed Thermotoga maritima
T33Q cleavage site selection affected Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-
Pyrobaculum aerophilum
-
-
-
Thermoplasma acidophilum
-
-
-
Thermotoga maritima
-
-
-

Synonyms

Synonyms Comment Organism
tRNase Z
-
Bacillus subtilis
tRNase Z
-
Escherichia coli
tRNase Z
-
Thermoplasma acidophilum
tRNase Z
-
Thermotoga maritima
tRNase Z
-
Pyrobaculum aerophilum