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Literature summary for 3.1.26.11 extracted from

  • Mohan, A.; Whyte, S.; Wang, X.; Nashimoto, M.; Levinger, L.
    The 3' end CCA of mature tRNA is an antideterminant for eukaryotic 3' -tRNase (1999), RNA, 5, 245-256.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
additional information the product of the 3'-tRNase reaction is a competitive inhibitor Drosophila melanogaster
additional information the product of the 3'-tRNase reaction is a competitive inhibitor Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00002
-
tRNA(His48) at 37°C Sus scrofa
0.00004
-
tRNA(His48) at 28°C Sus scrofa
0.00008
-
tRNA(His48)
-
Drosophila melanogaster
0.00009
-
tRNAHis48 3'+ CC CC is added to the 3'-end of tRNA Sus scrofa
0.00009
-
tRNA(His48) C is added to the 3'-end of tRNA Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ highest reaction velocity at 3 mM Sus scrofa
Mg2+ highest reaction velocity at 0.3-1 mM Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-
Sus scrofa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
t-RNAHis48 + H2O
-
Drosophila melanogaster ?
-
?
t-RNAHis48 + H2O
-
Sus scrofa ?
-
?
t-RNAHis48 3'C + H2O
-
Drosophila melanogaster ?
-
?
t-RNAHis48 3'C + H2O
-
Sus scrofa ?
-
?
t-RNAHis48 3'CC + H2O
-
Drosophila melanogaster ?
-
?
t-RNAHis48 3'CC + H2O
-
Sus scrofa ?
-
?
tRNA(His48) + H2O
-
Drosophila melanogaster ?
-
?
tRNA(His48) + H2O
-
Sus scrofa ?
-
?
tRNAHis48 3'+ C + H2O
-
Sus scrofa ?
-
?
tRNAHis48 3'+ CC + H2O
-
Sus scrofa ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
Drosophila melanogaster