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Literature summary for 3.1.22.5 extracted from

  • Ghosh, S.; DasGupta, U.
    Studies with endonuclease X: A new deoxyendonuclease of E. coli that preferentially cleaves supercoiled plasmid DNA (1984), Curr. Trends Life Sci., 12, 79-88.
No PubMed abstract available
Search for more literature for 3.1.22.5

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
 Escherichia coli
ATP
-
 Escherichia coli
Ca2+ at concentrations above 1.5 mM Escherichia coli
Mg2+ at concentrations above 5 mM Escherichia coli
additional information no effect: adenosine, thymidine, RNA and double-stranded DNA Escherichia coli
single-stranded DNA
-
 Escherichia coli
Tris-HCl optimum concentration at 10-150 mM, inhibition near 150 mM Escherichia coli
TTP
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ absolute requirement for a divalent cation, optimum concentration: 1.5 mM, inhibition at higher concentrations Escherichia coli
Mg2+ absolute requirement for a divalent cation, optimum concentration: 5 mM, inhibition at higher concentrations Escherichia coli
additional information very little activity with Mn2+ or Zn2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 x * 49000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
supercoiled plasma DNA + H2O Escherichia coli
-
 linear DNA duplexes
-
 ?
supercoiled plasma DNA + H2O Escherichia coli B / ATCC 11303
-
 linear DNA duplexes
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linear double-stranded DNA + H2O little activity Escherichia coli ?
-
 ?
linear double-stranded DNA + H2O little activity Escherichia coli B / ATCC 11303 ?
-
 ?
supercoiled plasma DNA + H2O
-
 Escherichia coli linear DNA duplexes
-
 ?
supercoiled plasma DNA + H2O enzyme recognizes cruziforme structure in supercoiled plasmid DNA, e.g. pBR322 form I DNA is first converted to relaxed circular form II-dimer and subsequently to linear form III-dimer of pBR322, ColE-1 DNA is converted to linear products, the enzyme appears to cleave at more than one site on superhelical pBR322 DNA, a double-strand break at one site seems to block further double-strand cleavage in the DNA molecule Escherichia coli linear DNA duplexes
-
 ?
supercoiled plasma DNA + H2O not: lambda DNA Escherichia coli linear DNA duplexes
-
 ?
supercoiled plasma DNA + H2O
-
 Escherichia coli B / ATCC 11303 linear DNA duplexes
-
 ?
supercoiled plasma DNA + H2O enzyme recognizes cruziforme structure in supercoiled plasmid DNA, e.g. pBR322 form I DNA is first converted to relaxed circular form II-dimer and subsequently to linear form III-dimer of pBR322, ColE-1 DNA is converted to linear products, the enzyme appears to cleave at more than one site on superhelical pBR322 DNA, a double-strand break at one site seems to block further double-strand cleavage in the DNA molecule Escherichia coli B / ATCC 11303 linear DNA duplexes
-
 ?
supercoiled plasma DNA + H2O not: lambda DNA Escherichia coli B / ATCC 11303 linear DNA duplexes
-
 ?

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.8 pBR322 Escherichia coli