Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.22.1 extracted from

  • Padron-Barthe, L.; Courta, J.; Lepretre, C.; Nagbou, A.; Torriglia, A.
    Leukocyte Elastase Inhibitor, the precursor of L-DNase II, inhibits apoptosis by interfering with caspase-8 activation (2008), Biochim. Biophys. Acta, 1783, 1755-1766.
    View publication on PubMed
Search for more literature for 3.1.22.1

Application

Application Comment Organism
medicine high expression of LEI in tumor cells may reduce the efficiency of etoposide as a chemotherapeutic agent. LEI inhibits cathepsin D, released from the lysosome during etoposide treatment. Cathepsin D enhances caspase activity in the cell by cleaving procaspase-8 and LEI overexpression slows down this cleavage, protecting cells from apoptosis Sus scrofa

Cloned(Commentary)

Cloned (Comment) Organism
wild-type or mutant LEI expressed in BHK-21 cells. 6xHis tagged wild-type and mutant LEI expressed from pET 23d(+) plasmid in Escherichia coli BL21(DE3)pLysS strain. Wild-type LEI expressed in HeLa cells Sus scrofa

Protein Variants

Protein Variants Comment Organism
AP10T point mutation in the hinge region of LEI, has decreased anti-protease activity. Cells overexpressing wild-type LEI or AP10T-LEI have the same survival rate under unstressed condition. Mutant retains its pro-apoptotic activity when transformed into L-DNase II by an apoptotic stimulus (e.g., hexa-methylene-amiloride treatment). When mutant cells are treated with etoposide they do not present the same survival rate than wild-type cells Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion fraction of procaspase-8 and LEI colocalise in the mitochondria. After etoposide treatment, cathepsin D is released from lysosomes and part of it colocalises with caspase-8 and LEI Sus scrofa 5739
-

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Synonyms

Synonyms Comment Organism
L-DNase II
-
 Sus scrofa
LEI
-
 Sus scrofa