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Literature summary for 3.1.22.1 extracted from
- Nuclear export of LEI/L-DNase II by Crm1 is essential for cell survival (2008), Biochim. Biophys. Acta, 1783, 1068-1075.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | a nuclear export signal on LEI involved in the control of LEI/L-DNase II nuclearization in healthy cells | Sus scrofa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| LEI cDNA subcloned into pZeoSV2+, into pET23d+ in frame with the His-tag, and into pDsRed2-C1 plasmid in frame with the red fluorescent protein fused to its N-term. Expression in Escherichia coli strain BL21(DE3) pLysS. BHK cells transfected with a pZeo vector containing the wild-type or a punctually mutated LEI cDNA | Sus scrofa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L286A | displays a decrease of survival compared to the wild-type | Sus scrofa |
| L290A | displays no decrease of survival compared to the wild-type | Sus scrofa |
| L293A | displays a decrease of survival compared to the wild-type | Sus scrofa |
| V295A | exhibits a significant cell death compared to the wild-type, highest rate of cells with nuclear retention of LEI | Sus scrofa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | LEI is concentrated around the nucleus | Homo sapiens | 5737 | - |
| cytoplasm | LEI is concentrated around the nucleus. LEI/L-DNase II nuclear export is leptomycin B sensitive. The export is mediated by Crm1 receptor | Cricetulus griseus | 5737 | - |
| additional information | putative leucine-rich nuclear export signal on LEI/L-DNase II sequence, a mandatory condition for binding exportin molecules. The exportin Crm1 interacts with LEI/L-DNase II | Sus scrofa | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus griseus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ARPE-19 cell | - |
Homo sapiens | - |
| HeLa cell | - |
Homo sapiens | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LEI-derived DNase II | - |
Cricetulus griseus |
| LEI-derived DNase II | - |
Homo sapiens |
| LEI-derived DNase II | - |
Sus scrofa |
| LEI/L-DNase II | - |
Cricetulus griseus |
| LEI/L-DNase II | - |
Homo sapiens |
| LEI/L-DNase II | - |
Sus scrofa |
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