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Literature summary for 3.1.21.9 extracted from

  • Hirano, N.; Ohshima, H.; Takahashi, H.
    Biochemical analysis of the substrate specificity and sequence preference of endonuclease IV from bacteriophage T4, a dC-specific endonuclease implicated in restriction of dC-substituted T4 DNA synthesis (2006), Nucleic Acids Res., 34, 4743-4751.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in wheat germ-free expression system Tequatrovirus T4

Inhibitors

Inhibitors Comment Organism Structure
KCl 50 mM, 20% residual activity Tequatrovirus T4
NaCl 50 mM, 15% residual activity Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 5'-(dA)8dGdTdCdAdG(dA)8-3' pH 8.0, 37°C Tequatrovirus T4
0.018
-
 5'-(dA)7dAdTdCdAdA(dA)7-3' pH 8.0, 37°C Tequatrovirus T4
0.023
-
 5'-(dA)7dAdAdCdAdA(dA)7-3' pH 8.0, 37°C Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ may substitute for Mg2+ Tequatrovirus T4
Co2+ may substitute for Mg2+ Tequatrovirus T4
Mg2+ optimum concentration 10 mM Tequatrovirus T4
Mn2+ may substitute for Mg2+ Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-(dA)7dAdAdCdAdA(dA)7-3' + H2O
-
 Tequatrovirus T4 ?
-
 ?
5'-(dA)7dAdTdCdAdA(dA)7-3' + H2O
-
 Tequatrovirus T4 ?
-
 ?
5'-(dA)8dGdTdCdAdG(dA)8-3' + H2O
-
 Tequatrovirus T4 ?
-
 ?
additional information enzyme shows high activity with single-stranded DNA and denatured dC-substituted T4 genomic double-stranded DNA but exhibits no activity with dsDNA, ssRNA or denatured T4 genomic dsDNA containing glucosylated deoxyhydroxymethylcytidine. Endo IV catalyzes specific endonucleolytic cleavage of the 5' phosphodiester bond of dC in ssDNA with an efficiency markedly dependent on the surrounding nucleotide sequence. The enzyme preferentially targets 5'-dTdCdA-3' but tolerates various combinations of individual nucleotides flanking this trinucleotide sequence Tequatrovirus T4 ?
-
 ?

Synonyms

Synonyms Comment Organism
denB
-
 Tequatrovirus T4

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00576
-
 5'-(dA)7dAdAdCdAdA(dA)7-3' pH 8.0, 37°C Tequatrovirus T4
0.0087
-
 5'-(dA)8dGdTdCdAdG(dA)8-3' pH 8.0, 37°C Tequatrovirus T4
0.0167
-
 5'-(dA)7dAdTdCdAdA(dA)7-3' pH 8.0, 37°C Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 9.2
-
 Tequatrovirus T4

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.05
-
 5'-(dA)8dGdTdCdAdG(dA)8-3' pH 8.0, 37°C Tequatrovirus T4
0.08
-
 5'-(dA)7dAdTdCdAdA(dA)7-3' pH 8.0, 37°C Tequatrovirus T4
0.25
-
 5'-(dA)7dAdAdCdAdA(dA)7-3' pH 8.0, 37°C Tequatrovirus T4