Literature summary for 3.1.21.5 extracted from

Butterer, A.; Pernstich, C.; Smith, R.M.; Sobott, F.; Szczelkun, M.D.; Toth, J.
Type III restriction endonucleases are heterotrimeric: comprising one helicase-nuclease subunit and a dimeric methyltransferase that binds only one specific DNA (2014), Nucleic Acids Res., 42, 5139-5150.

Search for more literature for 3.1.21.5

Activating Compound

Activating Compound	Comment	Organism	Structure
ATP	ATP hydrolysis activated by recognition site binding leads to release of the enzyme from the site, dissociation from the substrate via a free DNA end and cleavage of the DNA	Escherichia coli
ATP	ATP hydrolysis activated by recognition site binding leads to release of the enzyme from the site, dissociation from the substrate via a free DNA end and cleavage of the DNA	Providencia stuartii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on	Escherichia coli
Mg2+	dependent on	Providencia stuartii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
63715	-	1 * 108264 + 2 * 63715, calculated from amino acid sequence	Providencia stuartii
73486	-	1 * 111459 + 2 * 73486, EcoPI, calculated from amino acid sequence	Escherichia coli
74094	-	1 * 110957 + 2 * 74094, EcoP15I, calculated from amino acid sequence	Escherichia coli
108264	-	1 * 108264 + 2 * 63715, calculated from amino acid sequence	Providencia stuartii
110957	-	1 * 110957 + 2 * 74094, EcoP15I, calculated from amino acid sequence	Escherichia coli
111459	-	1 * 111459 + 2 * 73486, EcoPI, calculated from amino acid sequence	Escherichia coli
235700	-	calculated from amino acid sequence	Providencia stuartii
258400	-	EcoPI, calculated from amino acid sequence	Escherichia coli
259100	-	EcoP15I, calculated from amino acid sequence	Escherichia coli
259300	-	EcoP15I, multi-angle light scattering	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA + H2O	Escherichia coli	-	specific double-stranded DNA fragments with terminal 5'-phosphate	-	?
DNA + H2O	Providencia stuartii	-	specific double-stranded DNA fragments with terminal 5'-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Providencia stuartii	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	-	Escherichia coli	specific double-stranded DNA fragments with terminal 5'-phosphate	-	?
DNA + H2O	-	Providencia stuartii	specific double-stranded DNA fragments with terminal 5'-phosphate	-	?
additional information	type III restriction endonucleases comprise of one helicase-nuclease subunit and a dimeric methyltransferase that binds only one specific DNA	Escherichia coli	?	-	?
additional information	type III restriction endonucleases comprise of one helicase-nuclease subunit and a dimeric methyltransferase that binds only one specific DNA	Providencia stuartii	?	-	?

Subunits

Subunits	Comment	Organism
heterotrimer	1 * 108264 + 2 * 63715, calculated from amino acid sequence	Providencia stuartii
heterotrimer	1 * 110957 + 2 * 74094, EcoP15I, calculated from amino acid sequence	Escherichia coli
heterotrimer	1 * 111459 + 2 * 73486, EcoPI, calculated from amino acid sequence	Escherichia coli

Synonyms

Synonyms	Comment	Organism
EcoP15I	-	Escherichia coli
EcoPI	-	Escherichia coli
PstII	-	Providencia stuartii
type III restriction endonuclease	-	Escherichia coli
type III restriction endonuclease	-	Providencia stuartii

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Release 2023.1 (January 2023)