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Literature summary for 3.1.21.5 extracted from

  • Schwarz, F.W.; van Aelst, K.; Toth, J.; Seidel, R.; Szczelkun, M.D.
    DNA cleavage site selection by Type III restriction enzymes provides evidence for head-on protein collisions following 1D bidirectional motion (2011), Nucleic Acids Res., 39, 8042-8051.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
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Synonyms

Synonyms Comment Organism
EcoP15I
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Escherichia coli
EcoPI
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Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli

General Information

General Information Comment Organism
additional information sliding model for long-range communication on DNA by type III restriction enzymes, distribution of cleavage on head-to-head substrates with end bias, overview Escherichia coli
physiological function DNA cleavage by the type III restriction-modification enzymes requires communication in 1D between two distant indirectly-repeated recognitions sites, resulting in non-specific dsDNA cleavage close to only one of the two sites. The cleavage site selection reflects the dynamics of the preceding stochastic enzyme events that are consistent with bidirectional motion in 1D and DNA cleavage following head-on protein collision. The type III REs exhibits site orientation selectivity Escherichia coli