Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
EcoP15I | - |
Escherichia coli |
EcoPI | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
additional information | sliding model for long-range communication on DNA by type III restriction enzymes, distribution of cleavage on head-to-head substrates with end bias, overview | Escherichia coli |
physiological function | DNA cleavage by the type III restriction-modification enzymes requires communication in 1D between two distant indirectly-repeated recognitions sites, resulting in non-specific dsDNA cleavage close to only one of the two sites. The cleavage site selection reflects the dynamics of the preceding stochastic enzyme events that are consistent with bidirectional motion in 1D and DNA cleavage following head-on protein collision. The type III REs exhibits site orientation selectivity | Escherichia coli |