Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain ER2744 | Pyrococcus sp. GI-H |
Protein Variants | Comment | Organism |
---|---|---|
D138A | catalytically inactive | Pyrococcus sp. GI-H |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Pyrococcus sp. GI-H |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus sp. GI-H | - |
- |
- |
Purification (Comment) | Organism |
---|---|
P11 cellulose phosphate column chromatography | Pyrococcus sp. GI-H |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Pyrococcus sp. GI-H | specific double-stranded DNA fragments with terminal 5'-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PspGI | recognizes the sequence CCWGG where W is A or T and cuts DNA before the first C in the cognate sequence | Pyrococcus sp. GI-H |
REase | - |
Pyrococcus sp. GI-H |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
the optimal temperature for DNA cleavage by PspGI is at 80°C or above | Pyrococcus sp. GI-H |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
half life of 2 h at 95°C | Pyrococcus sp. GI-H |