Cloned (Comment) | Organism |
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- |
Providencia stuartii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Providencia stuartii | the intact Res2Mod2 tetramer is a fast endonuclease and slow methyltransferase, thereby favouring DNA cleavage, subassemblies of PstII in which the Res subunits have dissociated are more efficient methyltransferases. DNA cleavage by these lower molecular weight species may only occur if sufficient hsdR associates to form a Res2Mod2 tetramer before methylation occurs. This dynamic association of Res and Mod might play a key role in the control of PstII activity in vivo | specific double-stranded DNA fragments with terminal 5'-phosphate | - |
? | |
DNA + H2O | Providencia stuartii 164 | the intact Res2Mod2 tetramer is a fast endonuclease and slow methyltransferase, thereby favouring DNA cleavage, subassemblies of PstII in which the Res subunits have dissociated are more efficient methyltransferases. DNA cleavage by these lower molecular weight species may only occur if sufficient hsdR associates to form a Res2Mod2 tetramer before methylation occurs. This dynamic association of Res and Mod might play a key role in the control of PstII activity in vivo | specific double-stranded DNA fragments with terminal 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Providencia stuartii | - |
- |
- |
Providencia stuartii 164 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Providencia stuartii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Providencia stuartii | specific double-stranded DNA fragments with terminal 5'-phosphate | - |
? | |
DNA + H2O | the intact Res2Mod2 tetramer is a fast endonuclease and slow methyltransferase, thereby favouring DNA cleavage, subassemblies of PstII in which the Res subunits have dissociated are more efficient methyltransferases. DNA cleavage by these lower molecular weight species may only occur if sufficient hsdR associates to form a Res2Mod2 tetramer before methylation occurs. This dynamic association of Res and Mod might play a key role in the control of PstII activity in vivo | Providencia stuartii | specific double-stranded DNA fragments with terminal 5'-phosphate | - |
? | |
DNA + H2O | recognition sequence: CTGATG. The enzyme requires a substrate with two copies of the recognition site in head-to-head repeat and is dependent on a low level of ATP hydrolysis (about 40 ATP/site/min). PstII can cut DNA with GTP and CTP | Providencia stuartii | specific double-stranded DNA fragments with terminal 5'-phosphate | - |
? | |
DNA + H2O | - |
Providencia stuartii 164 | specific double-stranded DNA fragments with terminal 5'-phosphate | - |
? | |
DNA + H2O | the intact Res2Mod2 tetramer is a fast endonuclease and slow methyltransferase, thereby favouring DNA cleavage, subassemblies of PstII in which the Res subunits have dissociated are more efficient methyltransferases. DNA cleavage by these lower molecular weight species may only occur if sufficient hsdR associates to form a Res2Mod2 tetramer before methylation occurs. This dynamic association of Res and Mod might play a key role in the control of PstII activity in vivo | Providencia stuartii 164 | specific double-stranded DNA fragments with terminal 5'-phosphate | - |
? | |
DNA + H2O | recognition sequence: CTGATG. The enzyme requires a substrate with two copies of the recognition site in head-to-head repeat and is dependent on a low level of ATP hydrolysis (about 40 ATP/site/min). PstII can cut DNA with GTP and CTP | Providencia stuartii 164 | specific double-stranded DNA fragments with terminal 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | the intact Res2Mod2 tetramer is a fast endonuclease and slow methyltransferase, thereby favouring DNA cleavage, subassemblies of PstII in which the Res subunits have dissociated are more efficient methyltransferases. DNA cleavage by these lower molecular weight species may only occur if sufficient hsdR associates to form a Res2Mod2 tetramer before methylation occurs. This dynamic association of Res and Mod might play a key role in the control of PstII activity in vivo | Providencia stuartii |
Synonyms | Comment | Organism |
---|---|---|
PstII | - |
Providencia stuartii |
restriction endonuclease PstII | - |
Providencia stuartii |