Cloned (Comment) | Organism |
---|---|
gene bspRIR, cloning from R genomic DNA, expression in Escherichia coli, which is dependent on the replacement of the native TTG initiation codon with an ATG codon, method development, overview | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
gene bspRIR | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | usage of a plasmid containing a single BspRI recognition site to analyze kinetically nicking and second-strand cleavage under steady-state conditions. Cleavage of the supercoiled plasmid goes through a relaxed intermediate indicating sequential hydrolysis of the two strands. BspRI cleaves the two DNA strands sequentially | Lysinibacillus sphaericus | ? | - |
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Subunits | Comment | Organism |
---|---|---|
More | peptide mapping, tryptic digestion and mass spectrometry analysis, overview | Lysinibacillus sphaericus |
Synonyms | Comment | Organism |
---|---|---|
BspRI | - |
Lysinibacillus sphaericus |
GGCC-specific restriction endonuclease | - |
Lysinibacillus sphaericus |
More | BspRI and the related putative restriction endonucleases belong to the PD-(D/E)XK nuclease superfamily | Lysinibacillus sphaericus |
type IIP REase | - |
Lysinibacillus sphaericus |
type IIP restriction endonuclease | - |
Lysinibacillus sphaericus |
General Information | Comment | Organism |
---|---|---|
additional information | type IIP restriction endonucleases are characterized by recognition sequences displaying dyad axes of symmetry (palindromes), and constitute the most abundant class of characterized restriction enzymes | Lysinibacillus sphaericus |