Cloned (Comment) | Organism |
---|---|
expression in Escheichia coli, induction at 15°C results in accumulation in the cytoplasm, induction at 20-37°C results in formation of inclusion bodies | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme is an endonuclease encoded as a protein insert or intein within the yeast V-ATPase catalytic subunit encoding gene vma1. Insertion of the Green Fluorescence Protein into a loop which is located between the endonuclease and splicing domains of the Sce VMA1 intein. The GFP is functional and the additional GFP domain does not prevent intein excision and endonuclease activity. Contrary to wild-type, mutant requires the presence of Mn2+ and not Mg2+ ions for activity | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for wild-type activity | Saccharomyces cerevisiae | |
Mn2+ | required for activity of mutant which carries an insertion of the Green Fluorescence Protein into a loop which is located between the endonuclease and splicing domains of the Sce VMA1 intein | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
isoform SceI | - |