KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | determination of kinetic parameters for two-step DNA cleavage reactions by the enzyme using a DNA-immobilized 27 Mhz quartz crystal microbalance | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | site-specific binding of enzyme to DNA in presence of Ca2+, without the catalytic cleavage. Binding kinetic parameters in presence of Ca2+ are consistent with those in presence of Mg2+ | Escherichia coli | |
Mg2+ | site-specific binding of enzyme to DNA in presence of Mg2+, and site-specific cleavage reaction. Binding kinetic parameters in presence of Ca2+ are consistent with those in presence of Mg2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isoform EcoRV | - |