Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.21.4 extracted from

  • Vitkute, J.; Maneliene, Z.; Janulaitis, A.
    Two new thermostable type II restriction endonucleases from Thermus aquaticus: TatI and TauI, which recognize the novel nucleotide sequences 5'-W|GTACW-3' and 5'-GCSG|C-3' respectively (2001), FEMS Microbiol. Lett., 204, 253-257.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
enzymes TatI, TauI
-

Purification (Commentary)

Purification (Comment) Organism
partial Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information TatI recognition site is 5’-AGTACA-3’, the enzyme cleaves between first and second nucleotides generating 5’-ends protruding four bases, TauI recognition site is 5’-GCGSGC-3’, the enzyme cleaves between fourth and fifth nucleotides generating 3’-ends protruding three bases Thermus aquaticus ?
-
?
phage lambda DNA
-
Thermus aquaticus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
assay Thermus aquaticus