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Literature summary for 3.1.21.4 extracted from

  • Morgan, R.; Xiao, J.P.; Xu, S.Y.
    Characterization of an extremely thermostable restriction enzyme, PspGI, from a Pyrococcus strain and cloning of the PspGI restriction-modification system in Escherichia coli (1998), Appl. Environ. Microbiol., 64, 3669-3673.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.4

Application

Application Comment Organism
analysis the enzyme can be used in DNA-based diagnostic applications Pyrococcus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli via a T7 expression system Pyrococcus sp.

General Stability

General Stability Organism
enzyme remains active following 30 cycles of thermocycling Pyrococcus sp.

Organism

Organism UniProt Comment Textmining
Pyrococcus sp.
-
 enzyme PspGI
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pyrococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O cleaves DNA before the first C in the sequence 5'-CCWGG3'. W is A or T Pyrococcus sp. double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Synonyms

Synonyms Comment Organism
PspGI
-
 Pyrococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75 85
-
 Pyrococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 85 digestion of T7 DNA Pyrococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 half-life: 2 h Pyrococcus sp.