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Literature summary for 3.1.21.4 extracted from

  • Kapfer, W.; Walter, J.; Trautner, T.A.
    Cloning, characterization and evolution of the BsuFI restriction endonuclease gene of Bacillus subtilis and purification of the enzyme (1991), Nucleic Acids Res., 19, 6457-6463.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Bacillus subtilis
45600
-
 x * 45600, calculation from nucleotide sequence Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Bacillus subtilis primary function is the inactivation of foreign DNA invading bacteria ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 enzyme R.BsuFI
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Storage Stability

Storage Stability Organism
-20°C, stable for at least 6 months Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O the enzyme recognizes the target DNA sequence 5'CCGG and cleaves between the two cytosines to produce sticky ends with 5'CG overhangs Bacillus subtilis double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
DNA + H2O primary function is the inactivation of foreign DNA invading bacteria Bacillus subtilis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Bacillus subtilis
? x * 45600, calculation from nucleotide sequence Bacillus subtilis

Synonyms

Synonyms Comment Organism
R.BsuFI
-
 Bacillus subtilis