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Literature summary for 3.1.21.3 extracted from

  • Bogdanova, E.; Zakharova, M.; Streeter, S.; Taylor, J.; Heyduk, T.; Kneale, G.; Severinov, K.
    Transcription regulation of restriction-modification system Esp1396I (2009), Nucleic Acids Res., 37, :3354-3366.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.3

Application

Application Comment Organism
additional information transcription of both the restriction endonuclease and methyltransferase genes is controlled by interdependent regulatory loops governed by the C.Esp1396I protein synthetic construct

Cloned(Commentary)

Cloned (Comment) Organism
esp1396I genes expressed in Escherichia coli HB101 and XL1-Blue synthetic construct

Protein Variants

Protein Variants Comment Organism
additional information mutations within the tetranucleotide repeats diminish binding of C.Esp1396I. Insertion of an additional base into the TATA sequence to make the GACT/AGCT sequences symmetric around a TAT spacer reduces DNA binding synthetic construct

Inhibitors

Inhibitors Comment Organism Structure
additional information a repressing complex of C.Esp1396I tetramer bound to both binding sites synthetic construct

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17920
-
 sedimentation equilibrium analytical ultracentrifugation of C.Esp1396I synthetic construct
18450
-
 theoretical Mr of C.Esp1396I synthetic construct

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information synthetic construct basal transcription from promoter esp1396ICR results in gradual accumulation of C.Esp1396I, which upon dimerization binds to a single site in promoter esp1396IM, preventing further transcription from this promoter. Further accumulation of C.Esp1396I results in activation and then gradual repression of promoter esp1396ICR ?
-
 ?

Organism

Organism UniProt Comment Textmining
synthetic construct
-
-
-

Purification (Commentary)

Purification (Comment) Organism
C.Esp1396I-6His and C.Esp1396I without hexahistidine tag synthetic construct

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 synthetic construct ?
-
 ?
additional information basal transcription from promoter esp1396ICR results in gradual accumulation of C.Esp1396I, which upon dimerization binds to a single site in promoter esp1396IM, preventing further transcription from this promoter. Further accumulation of C.Esp1396I results in activation and then gradual repression of promoter esp1396ICR synthetic construct ?
-
 ?

Subunits

Subunits Comment Organism
dimer sedimentation equilibrium analytical ultracentrifugation of C.Esp1396I synthetic construct

Synonyms

Synonyms Comment Organism
Esp1396I
-
 synthetic construct