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Literature summary for 3.1.21.3 extracted from

  • Weiserova, M.; Ryu, J.
    Characterization of a restriction modification system from the commensal Escherichia coli strain A0 34/86 (O83:K24:H31) (2008), BMC Microbiol., 8, 106.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.3

Application

Application Comment Organism
medicine identification of a putative restriction-modification system EcoA0ORF42P in the commensal Escherichia coli strain A0 34/86 (O83: K24: H31), which is efficiently used at Czech paediatric clinics for prophylaxis and treatment of nosocomial infections and diarrhoea of preterm and newborn infants. This type I R-M system is a member of the type IB family, but is not an isoschizomer of either any prototype of the type IB members or any sequenced putative IB R-M systems. It is designated as EcoAO83I, the DNA recognition sequence of the EcoAO83I is GGA(8N)ATGC. Combination of the classical biochemical and bacterial genetics approaches with comparative genomics may contribute effectively to further classification of many other putative type-I enzymes, especially in clinical samples Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
transforming the Escherichia coli BL21(DE3) strains with a BAC C4/1 carrying the hsdR, hsdM and hsdS genes of EcoAO83I and with plasmids carrying the hsdS and hsdM genes of EcoAI Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli Q07736
-
-
Escherichia coli Q47281
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-
Escherichia coli A0 34/86 (O83:K24:H31)
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-
-
Escherichia coli A0 34/86 (O83:K24:H31) Q07736
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-
Escherichia coli A0 34/86 (O83:K24:H31) Q47281
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
duplex DNA + ATP
-
 Escherichia coli double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
 ?
duplex DNA + ATP
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 Escherichia coli A0 34/86 (O83:K24:H31) double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
 ?

Synonyms

Synonyms Comment Organism
EcoA0ORF42P
-
 Escherichia coli
EcoAI
-
 Escherichia coli
EcoAO83I
-
 Escherichia coli
EcoEI
-
 Escherichia coli
type I R-M system
-
 Escherichia coli
type I restriction-modification system
-
 Escherichia coli
type IB restriction enzyme
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli