Literature summary for 3.1.21.3 extracted from

Veiga, H.; Pinho, M.G.
Inactivation of the Sau1 Type I restriction-modification system is not sufficient to generate Staphylococcus aureus strains capable of efficiently accepting foreign DNA (2009), Appl. Environ. Microbiol., 75, 3034-3038.

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Application

Application	Comment	Organism
additional information	inactivation of the hsdR gene of the Sau1 type I restriction-modification system is responsible for the high transformation efficiency of RN4220	Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment)	Organism
hsdR PCR product cloned into pMAD, giving rise to pDELTAHsdR-1. Plasmid electroporated into the transformable strain RN4220 at 30°C, with erythromycin selection, and subsequently transduced to NCTC8325-4, SH1000 and COL using phage 80alpha	Staphylococcus aureus

Protein Variants

Protein Variants	Comment	Organism
additional information	hsdR null mutants, complete deletion of the hsdR gene is not sufficient to generate readily transformable NCTC8325-4, SH1000 and COL strains. HsdR mutant strains have growth rates identical to the parental strains and and are competent if transformed with appropriately modified Staphylococcus aureus DNA. HsdR mutants are competent but do not accept foreign DNA due to the presence of additional factor(s) that degrade unmodified DNA. Heat treatment of competent cells increases transformation efficiency of hsdR mutants	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	strain NCTC8325-4, strain SH1000, strain COL and strain 879R4RF	-

Synonyms

Synonyms	Comment	Organism
Sau1 type I restriction-modification system	-	Staphylococcus aureus

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Release 2023.1 (January 2023)