Cloned (Comment) | Organism |
---|---|
wild-type R.PspGI and its mutant expressed as pET21a-PspGI-WT and pET21a-PspGI-D138A in Escherichia coli strain ER2744 | Pyrococcus sp. |
Protein Variants | Comment | Organism |
---|---|---|
D138A | catalytically inactive | Pyrococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type R.PspGI and its mutant purified by chromatography, to homogeneity | Pyrococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | the PspGI restriction-modification system recognizes the sequence CCWGG. R.PspGI cuts DNA before the first C in the cognate sequence and M.PspGI methylates N4 of one of the cytosines in the sequence. R.PspGI flips both bases in the central base pair out of the duplex | Pyrococcus sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PspGI endonuclease | - |
Pyrococcus sp. |
PspGI restriction-modification system | - |
Pyrococcus sp. |
R.PspGI | - |
Pyrococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
optimal temperature for DNA cleavage. Optimal flipping occurs at temperatures substantially below the growth temperature of the source organism for PspGI and for the catalytic activity of endonuclease | Pyrococcus sp. |