Literature summary for 3.1.21.3 extracted from

Janscak, P.; Dryden, D.T.F.; Firman, K.
Analysis of the subunit assembly of the typeIC restriction-modification enzyme EcoR124I (1998), Nucleic Acids Res., 26, 4439-4445.

Search for more literature for 3.1.21.3

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	EcoR124I	-

Purification (Commentary)

Purification (Comment)	Organism
mixture of two enzyme species, the larger species has the stoichiometry R2M2S1, the smaller species has the stoichiometry of R1M2S1.only the R2M2S1 complex is capable of DNA cleavage, the R1M2S1 complex retains ATPase activity	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
duplex DNA + ATP	-	Escherichia coli	double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate	-	?

Subunits

Subunits	Comment	Organism
More	two enzyme species, the larger species has the stoichiometry R2M2S1, the smaller species has the stoichiometry of R1M2S1. Only the R2M2S1 complex is capable of DNA cleavage, the R1M2S1 complex retains ATPase activity. The HsdS subunit determines specificity, the HsdM subunit is responsible for DNA methylation, the HsdR subunit is required for restriction. The HsdM subunit and the HsdS subunit can also form an independent DNA methyltransferase with a subunit stoichiometry of M2S1	Escherichia coli

Synonyms

Synonyms	Comment	Organism
EcoR124I	-	Escherichia coli

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Release 2023.1 (January 2023)