Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(lambdaDE3) cells | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | complete inhibition at 50 mM | Mycobacterium tuberculosis | |
EDTA | complete inhibition at 50 mM | Mycobacterium tuberculosis | |
additional information | the enzyme is tolerant to EDTA up to 2 mM | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000691 | - |
5'-end-labeled 29 nucleotide double-stranded DNA | at pH 8.0 and 37°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates activity | Mycobacterium tuberculosis | |
Mg2+ | maximally stimulated in the presence of Mg2+ | Mycobacterium tuberculosis | |
Mn2+ | slight stimulation | Mycobacterium tuberculosis | |
additional information | not stimulated by Zn2+, Co2+ | Mycobacterium tuberculosis | |
additional information | the enzyme shows metal ion independent 3'->5' exonuclease activity | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26800 | - |
x * 26800, SDS-PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WQ13 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WQ13 | - |
- |
Purification (Comment) | Organism |
---|---|
Streptactin column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-end-labeled 19 nucleotide single-stranded DNA + H2O | - |
Mycobacterium tuberculosis | ? | - |
? | |
5'-end-labeled 19 nucleotide single-stranded DNA + H2O | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
5'-end-labeled 29 nucleotide double-stranded DNA + H2O | 92% cleavage after 30 min | Mycobacterium tuberculosis | ? | - |
? | |
5'-end-labeled 29 nucleotide double-stranded DNA + H2O | 92% cleavage after 30 min | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | endonuclease activity of the enzyme is about 2fold higher for double-stranded DNA in comparison to single-stranded DNA | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the enzyme also shows 3'->5' exonuclease activity | Mycobacterium tuberculosis | ? | - |
? | |
additional information | endonuclease activity of the enzyme is about 2fold higher for double-stranded DNA in comparison to single-stranded DNA | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | the enzyme also shows 3'->5' exonuclease activity | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26800, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
End | - |
Mycobacterium tuberculosis |
endonuclease IV | - |
Mycobacterium tuberculosis |
Rv0670 | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0193 | - |
5'-end-labeled 29 nucleotide double-stranded DNA | at pH 8.0 and 37°C | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays a crucial role in defense against oxidative stress | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2790 | - |
5'-end-labeled 29 nucleotide double-stranded DNA | at pH 8.0 and 37°C | Mycobacterium tuberculosis |