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Literature summary for 3.1.21.2 extracted from

  • Puri, R.V.; Singh, N.; Gupta, R.K.; Tyagi, A.K.
    Endonuclease IV Is the major apurinic/apyrimidinic endonuclease in Mycobacterium tuberculosis and is important for protection against oxidative damage (2013), PLoS ONE, 8, e71535.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(lambdaDE3) cells Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol complete inhibition at 50 mM Mycobacterium tuberculosis
EDTA complete inhibition at 50 mM Mycobacterium tuberculosis
additional information the enzyme is tolerant to EDTA up to 2 mM Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00000691
-
5'-end-labeled 29 nucleotide double-stranded DNA at pH 8.0 and 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates activity Mycobacterium tuberculosis
Mg2+ maximally stimulated in the presence of Mg2+ Mycobacterium tuberculosis
Mn2+ slight stimulation Mycobacterium tuberculosis
additional information not stimulated by Zn2+, Co2+ Mycobacterium tuberculosis
additional information the enzyme shows metal ion independent 3'->5' exonuclease activity Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26800
-
x * 26800, SDS-PAGE Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WQ13
-
-
Mycobacterium tuberculosis H37Rv P9WQ13
-
-

Purification (Commentary)

Purification (Comment) Organism
Streptactin column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-end-labeled 19 nucleotide single-stranded DNA + H2O
-
Mycobacterium tuberculosis ?
-
?
5'-end-labeled 19 nucleotide single-stranded DNA + H2O
-
Mycobacterium tuberculosis H37Rv ?
-
?
5'-end-labeled 29 nucleotide double-stranded DNA + H2O 92% cleavage after 30 min Mycobacterium tuberculosis ?
-
?
5'-end-labeled 29 nucleotide double-stranded DNA + H2O 92% cleavage after 30 min Mycobacterium tuberculosis H37Rv ?
-
?
additional information endonuclease activity of the enzyme is about 2fold higher for double-stranded DNA in comparison to single-stranded DNA Mycobacterium tuberculosis ?
-
?
additional information the enzyme also shows 3'->5' exonuclease activity Mycobacterium tuberculosis ?
-
?
additional information endonuclease activity of the enzyme is about 2fold higher for double-stranded DNA in comparison to single-stranded DNA Mycobacterium tuberculosis H37Rv ?
-
?
additional information the enzyme also shows 3'->5' exonuclease activity Mycobacterium tuberculosis H37Rv ?
-
?

Subunits

Subunits Comment Organism
? x * 26800, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
End
-
Mycobacterium tuberculosis
endonuclease IV
-
Mycobacterium tuberculosis
Rv0670
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0193
-
5'-end-labeled 29 nucleotide double-stranded DNA at pH 8.0 and 37°C Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function the enzyme plays a crucial role in defense against oxidative stress Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2790
-
5'-end-labeled 29 nucleotide double-stranded DNA at pH 8.0 and 37°C Mycobacterium tuberculosis