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Literature summary for 3.1.21.2 extracted from

  • Sadowski, P.D.; Hurwitz, J.
    Enzymatic breakage of deoxyribonucleic acid. II. Purification and properties of endonuclease IV from T4 phage-infected Escherichia coli (1969), J. Biol. Chem., 244, 6192-6198.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slight activation Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 0.05 M Escherichia coli
KCl 0.05 M Escherichia coli
NaCl 0.05 M Escherichia coli
NH4Cl 0.05 M Escherichia coli
p-hydroxymercuribenzoate
-
Escherichia coli
potassium phosphate buffer pH 8.3 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 8% of the activity found compared to optimal Mg2+ concentration Escherichia coli
CoCl2 can substitute for MgCl2, optimal concentration: 0.01 M, activity 27% higher than at optimal MgCl2 concentration Escherichia coli
MgCl2 absolute requirement, no activity in absence, optimal concentration: 0.01 M Escherichia coli
Mn2+ 0.01 M can replace Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
single-stranded DNA + H2O Escherichia coli breakdown of host cell DNA occuring after T4 phage infection 5'-phosphooligonucleotides
-
ir
single-stranded DNA + H2O Escherichia coli B / ATCC 11303 breakdown of host cell DNA occuring after T4 phage infection 5'-phosphooligonucleotides
-
ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
phage T4 infected
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phage T4 infected Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
single-stranded DNA + H2O preference for single-stranded DNA Escherichia coli 5'-phosphooligonucleotides about 150 nucleotides long ir
single-stranded DNA + H2O denatured lambda-DNA, breaks exlusively adjacent to cytosine residues Escherichia coli 5'-phosphooligonucleotides about 150 nucleotides long ir
single-stranded DNA + H2O no activity on denatured T4 DNA Escherichia coli 5'-phosphooligonucleotides about 150 nucleotides long ir
single-stranded DNA + H2O breakdown of host cell DNA occuring after T4 phage infection Escherichia coli 5'-phosphooligonucleotides
-
ir
single-stranded DNA + H2O preference for single-stranded DNA Escherichia coli B / ATCC 11303 5'-phosphooligonucleotides about 150 nucleotides long ir
single-stranded DNA + H2O denatured lambda-DNA, breaks exlusively adjacent to cytosine residues Escherichia coli B / ATCC 11303 5'-phosphooligonucleotides about 150 nucleotides long ir
single-stranded DNA + H2O no activity on denatured T4 DNA Escherichia coli B / ATCC 11303 5'-phosphooligonucleotides about 150 nucleotides long ir
single-stranded DNA + H2O breakdown of host cell DNA occuring after T4 phage infection Escherichia coli B / ATCC 11303 5'-phosphooligonucleotides
-
ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 9.2
-
Escherichia coli