Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slight activation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 0.05 M | Escherichia coli | |
KCl | 0.05 M | Escherichia coli | |
NaCl | 0.05 M | Escherichia coli | |
NH4Cl | 0.05 M | Escherichia coli | |
p-hydroxymercuribenzoate | - |
Escherichia coli | |
potassium phosphate buffer | pH 8.3 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 8% of the activity found compared to optimal Mg2+ concentration | Escherichia coli | |
CoCl2 | can substitute for MgCl2, optimal concentration: 0.01 M, activity 27% higher than at optimal MgCl2 concentration | Escherichia coli | |
MgCl2 | absolute requirement, no activity in absence, optimal concentration: 0.01 M | Escherichia coli | |
Mn2+ | 0.01 M can replace Mg2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
single-stranded DNA + H2O | Escherichia coli | breakdown of host cell DNA occuring after T4 phage infection | 5'-phosphooligonucleotides | - |
ir | |
single-stranded DNA + H2O | Escherichia coli B / ATCC 11303 | breakdown of host cell DNA occuring after T4 phage infection | 5'-phosphooligonucleotides | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
phage T4 infected | - |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phage T4 infected | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
single-stranded DNA + H2O | preference for single-stranded DNA | Escherichia coli | 5'-phosphooligonucleotides | about 150 nucleotides long | ir | |
single-stranded DNA + H2O | denatured lambda-DNA, breaks exlusively adjacent to cytosine residues | Escherichia coli | 5'-phosphooligonucleotides | about 150 nucleotides long | ir | |
single-stranded DNA + H2O | no activity on denatured T4 DNA | Escherichia coli | 5'-phosphooligonucleotides | about 150 nucleotides long | ir | |
single-stranded DNA + H2O | breakdown of host cell DNA occuring after T4 phage infection | Escherichia coli | 5'-phosphooligonucleotides | - |
ir | |
single-stranded DNA + H2O | preference for single-stranded DNA | Escherichia coli B / ATCC 11303 | 5'-phosphooligonucleotides | about 150 nucleotides long | ir | |
single-stranded DNA + H2O | denatured lambda-DNA, breaks exlusively adjacent to cytosine residues | Escherichia coli B / ATCC 11303 | 5'-phosphooligonucleotides | about 150 nucleotides long | ir | |
single-stranded DNA + H2O | no activity on denatured T4 DNA | Escherichia coli B / ATCC 11303 | 5'-phosphooligonucleotides | about 150 nucleotides long | ir | |
single-stranded DNA + H2O | breakdown of host cell DNA occuring after T4 phage infection | Escherichia coli B / ATCC 11303 | 5'-phosphooligonucleotides | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 9.2 | - |
Escherichia coli |