Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Potyvirus sp. |
Protein Variants | Comment | Organism |
---|---|---|
C151A | activity similar to wild type | Potyvirus sp. |
D81G | no activity | Potyvirus sp. |
D81N | 50% activity compared to wild type | Potyvirus sp. |
H46A | activity similar to wild type | Potyvirus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibition observed above 50 microM, complete inhibition at 0.5 mM | Potyvirus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | - |
Potyvirus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Potyvirus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography and gel filtration, highly pure protein was used for assays | Potyvirus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Potyvirus sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded circular DNA + H2O | - |
Potyvirus sp. | ? | - |
? | |
double-stranded linear DNA + H2O | - |
Potyvirus sp. | ? | - |
? | |
lambda phage DNA + H2O | - |
Potyvirus sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Potyviral Ia Proeinase | - |
Potyvirus sp. |
PVBV proteinase | - |
Potyvirus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0214 | - |
lambda phage DNA | 37°C, 20 mM Tris-HCl pH 8.3, 1 mM MgCl2 | Potyvirus sp. | |
0.0214 | - |
(25R)-3beta-hydroxycholest-5-en-27-oate | 37°C, 20 mM Tris-HCl pH 8.3, 1 mM MgCl2 | Potyvirus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | - |
best activity above pH 8.0 | Potyvirus sp. |