Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Choristoneura fumiferana | |
K+ | no inhibition at 50 mM, 50% inhibition at 200 mM | Choristoneura fumiferana | |
Na+ | no inhibition at 50 mM, 50% inhibition at 200 mM | Choristoneura fumiferana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Choristoneura fumiferana | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, transition state metal ions also activate, but are less effective | Choristoneura fumiferana | |
Mg2+ | best activator, transition state metal ions also activate, but are less effective | Choristoneura fumiferana | |
additional information | divalent alkaline metal ion requirement | Choristoneura fumiferana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 23000, SDS-PAGE | Choristoneura fumiferana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsDNA + H2O | Choristoneura fumiferana | - |
5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
additional information | Choristoneura fumiferana | enzyme is an integral component of the larval digestive system, enzyme plays an important role in the conversion of the insecticidal crystal protein from Bacillus thuringiensis to the active DNA-free toxin in the larval gut, purified enzyme acts synergistically with the toxin | ? | - |
? | |
ssDNA + H2O | Choristoneura fumiferana | - |
5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Choristoneura fumiferana | - |
spruce budworm | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Choristoneura fumiferana |
Purification (Comment) | Organism |
---|---|
from larval gut | Choristoneura fumiferana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products | preference for single-stranded DNA | Choristoneura fumiferana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gut | - |
Choristoneura fumiferana | - |
larva | - |
Choristoneura fumiferana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsDNA + H2O | - |
Choristoneura fumiferana | 5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
additional information | no activity with homo-oligomers of dA, dC, dG and annealed homo-oligomers of dA/dT, no activity with RNA | Choristoneura fumiferana | ? | - |
? | |
additional information | enzyme is an integral component of the larval digestive system, enzyme plays an important role in the conversion of the insecticidal crystal protein from Bacillus thuringiensis to the active DNA-free toxin in the larval gut, purified enzyme acts synergistically with the toxin | Choristoneura fumiferana | ? | - |
? | |
ssDNA + H2O | - |
Choristoneura fumiferana | 5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
ssDNA + H2O | preferred substrate | Choristoneura fumiferana | 5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE | Choristoneura fumiferana |
Synonyms | Comment | Organism |
---|---|---|
DNase I | - |
Choristoneura fumiferana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | 11 | - |
Choristoneura fumiferana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9.5 | 12 | pH activity profile | Choristoneura fumiferana |