Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E128W | point mutation by sequential PCR steps, altered secondary structure, 80% reduced activity compared to the wild-type enzyme | Homo sapiens |
H84C | point mutation by sequential PCR steps, very slightly increased activity | Homo sapiens |
H84W | point mutation by sequential PCR steps, altered secondary structure, very slightly increased activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | inhibitory at concentrations above 80 mM | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, can be replaced by Mn2+ | Homo sapiens | |
Mn2+ | dependent on, can be replaced by Mg2+ | Homo sapiens | |
NaCl | activates at concentrations up to 40 mM | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products | Glu128 is required for activity, contains conserved sequence LEDFXR, endonuclease activity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tear | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Homo sapiens | 5'-phosphodinucleotides + 5'-phosphooligonucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme is of the Serratia marcescens type | Homo sapiens |
tear lipocalin | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |