Literature summary for 3.1.21.1 extracted from

Yusifov, T.N.; Abduragimov, A.R.; Gasymov, O.K.; Glasgow, B.J.
Endonuclease activity in lipocalins (2000), Biochem. J., 347, 815-819.

No PubMed abstract available

Search for more literature for 3.1.21.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant enzymes in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E128W	point mutation by sequential PCR steps, altered secondary structure, 80% reduced activity compared to the wild-type enzyme	Homo sapiens
H84C	point mutation by sequential PCR steps, very slightly increased activity	Homo sapiens
H84W	point mutation by sequential PCR steps, altered secondary structure, very slightly increased activity	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
NaCl	inhibitory at concentrations above 80 mM	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Homo sapiens	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on, can be replaced by Mn2+	Homo sapiens
Mn2+	dependent on, can be replaced by Mg2+	Homo sapiens
NaCl	activates at concentrations up to 40 mM	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products	Glu128 is required for activity, contains conserved sequence LEDFXR, endonuclease activity	Homo sapiens	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
tear	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	-	Homo sapiens	5'-phosphodinucleotides + 5'-phosphooligonucleotides	-	?

Synonyms

Synonyms	Comment	Organism
More	enzyme is of the Serratia marcescens type	Homo sapiens
tear lipocalin	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)