Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Nitro-5-thiocyanobenzoic acid | inhibition at identical rates | Bos taurus | |
2-Nitro-5-thiocyanobenzoic acid | inhibition at identical rates | Rattus sp. | |
EDTA | - |
Bos taurus | |
EDTA | - |
Rattus sp. | |
G-actin | - |
Bos taurus | |
G-actin | - |
Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Double-stranded DNA | 20 microgram/ml | Bos taurus | |
additional information | - |
Double-stranded DNA | 25 microgram/ml | Rattus sp. | |
additional information | - |
Double-stranded DNA | values in microgram DNA/ml | Bos taurus | |
additional information | - |
Double-stranded DNA | values in microgram DNA/ml | Rattus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation at 0.1 mM | Bos taurus | |
Ca2+ | activation at 0.1 mM | Rattus sp. | |
Ca2+ | activation synergistic with Mg2+ | Bos taurus | |
Ca2+ | activation synergistic with Mg2+ | Rattus sp. | |
Mg2+ | 5% activation | Bos taurus | |
Mg2+ | 5% activation | Rattus sp. | |
Mg2+ | activation synergistic with Ca2+ | Bos taurus | |
Mg2+ | activation synergistic with Ca2+ | Rattus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
SDS-PAGE | Rattus sp. |
34000 | - |
SDS-PAGE | Bos taurus |
34000 | - |
SDS-PAGE | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Bos taurus | preference for double-stranded DNA | 5'-phosphooligonucleotides + ? | - |
? | |
double-stranded DNA + H2O | Rattus sp. | preference for double-stranded DNA | 5'-phosphooligonucleotides + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rattus sp. | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Rattus sp. |
glycoprotein | DNase A with a neutral carbohydrate side-chain, DNase B with a sialic acid carbohydrate side-chain | Bos taurus |
Purification (Comment) | Organism |
---|---|
high homogeneity by chromatography steps | Bos taurus |
high homogeneity by chromatography steps | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
parotid gland | - |
Bos taurus | - |
parotid gland | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
units defined as absorbance at 260 nm/ml assay solution | Bos taurus |
additional information | - |
units defined as absorbance at 260 nm/ml assay solution | Rattus sp. |
additional information | - |
222 units/mg protein | Rattus sp. |
additional information | - |
938 units/mg protein | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Bos taurus | 5'-phosphooligonucleotides + ? | - |
? | |
DNA + H2O | - |
Rattus sp. | 5'-phosphooligonucleotides + ? | - |
? | |
DNA + H2O | no preference for any nucleotide | Bos taurus | 5'-phosphotrinucleotides + ? | as the main products | ? | |
DNA + H2O | no preference for any nucleotide | Rattus sp. | 5'-phosphotrinucleotides + ? | as the main products | ? | |
double-stranded DNA + H2O | preference for double-stranded DNA | Bos taurus | 5'-phosphooligonucleotides + ? | - |
? | |
double-stranded DNA + H2O | preference for double-stranded DNA | Rattus sp. | 5'-phosphooligonucleotides + ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Bos taurus |
7.8 | - |
- |
Rattus sp. |
8.2 | - |
- |
Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
activity completely lost | Bos taurus |
4 | - |
activity completely lost | Rattus sp. |