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Literature summary for 3.1.21.1 extracted from

  • Pan, C.Q.; Lazarus, R.A.
    Engineering hyperactive variants of human deoxyribonuclease I by altering its functional mechanism (1997), Biochemistry, 36, 6624-6632.
    View publication on PubMed
Search for more literature for 3.1.21.1

Application

Application Comment Organism
medicine inhaled DNase I into the airways reduces viscoelasticity of sputum, improves lung function of cystic fibrosis patients Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in human 293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
Q9R/E13R/N74K most hyperactive variant, 35fold more active than wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NaCl 50% inhibition at 60 mM, mutated enzyme less sensitive against increased salt concentrations Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation synergistic with Mg2+ Homo sapiens
Mg2+ activation synergistic with Ca2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
190mer DNA fragment + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
linearized plasmid-DNA + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + H2O
-
 ?
p-nitrophenyl phenylphosphonate + H2O
-
 Homo sapiens p-nitrophenol + phenylphosphoric acid
-
 ?
supercoiled plasmid DNA + H2O
-
 Homo sapiens linear DNA + ? formation of a relaxed circle that subsequently converts to linear DNA if it is nicked on the other strand at the same position ?