Application | Comment | Organism |
---|---|---|
medicine | inhaled DNase I into the airways reduces viscoelasticity of sputum, improves lung function of cystic fibrosis patients | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
overexpression in Sf9 cells as a DNaseI-Fc fusion- and a non-fusion protein | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | 10fold lower activity at 150 mM | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation synergistic with Mg2+ | Homo sapiens | |
Mg2+ | activation synergistic with Ca2+ | Homo sapiens | |
Mn2+ | employs more of the cutting mechanism of supercoiled plasmid-DNA | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Homo sapiens | predominantly by a single-stranded nicking mechanism in the presence of both Ca2+ and Mg2+ | 5'-phosphooligonucleotides + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
overexpressed DNaseI-Fc fusion protein | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.008 | - |
as plasmid nicking activities in the presence of both Mg2+ and Ca2+ for a DNaseI-Fc fusion protein | Homo sapiens |
0.096 | - |
as plasmid nicking activities in the presence of both Mg2+ and Ca2+ | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | predominantly by a single-stranded nicking mechanism in the presence of both Ca2+ and Mg2+ | Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
linearized plasmid-DNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + H2O | - |
? | |
p-nitrophenyl phenylphosphonate + H2O | - |
Homo sapiens | p-nitrophenol + phenylphosphoric acid | - |
? | |
supercoiled plasmid DNA + H2O | - |
Homo sapiens | linear DNA + ? | formation of a relaxed circle that subsequently converts to linear DNA if it is nicked on the other strand at the same position | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, SDS-PAGE | Homo sapiens |