Literature summary for 3.1.2.4 extracted from

Ibdah, M.; Pichersky, E.
Arabidopsis Chy1 null mutants are deficient in benzoic acid-containing glucosinolates in the seeds (2009), Plant Biol., 11, 574-581.

Search for more literature for 3.1.2.4

Cloned(Commentary)

Cloned (Comment)	Organism
open-reading frame of the Chy1 gene amplified, cDNA ligated into the vector pEX5-CT/TOPO TA to create a fusion of the open-reading frame with a His tag-coding extension at the C-terminus. Plasmid transferred into Escherichia coli BL21 (DE3) cells for expression	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	at 1 mM causes more than 90% inhibition of activity	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0029	-	cinnamoyl-CoA	at 30°C, in 50 mM Tris/HCl buffer, pH 7.0	Arabidopsis thaliana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	Mg2+, Fe2+, Cu2+, Zn2+, and Mn2+ at concentrations of 1 mM have no effect on the hydrolase activity	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9LKJ1	-	-

Purification (Commentary)

Purification (Comment)	Organism
by Ni chromatography	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-hydroxy-3-phenylpropionyl-CoA + H2O	-	Arabidopsis thaliana	3-hydroxy-3-phenylpropionate + CoA	-	?
cinnamoyl-CoA + H2O	-	Arabidopsis thaliana	cinnamic acid + CoA	-	?
additional information	has no hydrolase activity with benzoyl-CoA	Arabidopsis thaliana	?	-	?
p-coumaroyl-CoA + H2O	-	Arabidopsis thaliana	p-coumaric acid + CoA	-	?

Synonyms

Synonyms	Comment	Organism
CHY1	-	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	in 50 mm Tris-buffer	Arabidopsis thaliana

General Information

General Information	Comment	Organism
malfunction	mutations in the Chy1 gene lead to a deficiency of benzoic acid-containing glucosinolates in the seeds	Arabidopsis thaliana

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Release 2023.1 (January 2023)