Literature summary for 3.1.2.23 extracted from

Zhuang, Z.; Song, F.; Zhang, W.; Taylor, K.; Archambault, A.; Dunaway-Mariano, D.; Dong, J.; Carey, P.R.
Kinetic, Raman, NMR, and site-directed mutagenesis studies of the Pseudomonas sp. strain CBS3 4-hydroxybenzoyl-CoA thioesterase active site (2002), Biochemistry, 41, 11152-11160.

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Protein Variants

Protein Variants	Comment	Organism
D17E	site-directed mutagensis, increased activity	Pseudomonas sp.
D17N	site-directed mutagensis, highly reduced activity	Pseudomonas sp.
D17S	site-directed mutagensis, highly reduced activity	Pseudomonas sp.
K90A	site-directed mutagensis, enhanced sensitivity against inhibitor 4-hydroxyphenacyl-CoA	Pseudomonas sp.
R126L	site-directed mutagensis, slightly reduced activity	Pseudomonas sp.
R128A	site-directed mutagensis, similar to the wild-type enzyme	Pseudomonas sp.
R88A	site-directed mutagensis, slightly reduced activity	Pseudomonas sp.
R89L	site-directed mutagensis, similar to the wild-type enzyme	Pseudomonas sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
4-hydroxyphenacyl-CoA	-	Pseudomonas sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00014	-	4-hydroxybenzoyl-CoA	mutant K90A, pH 7.5, 25°C	Pseudomonas sp.
0.0024	-	4-hydroxybenzoyl-CoA	mutant D17E, pH 7.5, 25°C	Pseudomonas sp.
0.0045	-	4-hydroxybenzoyl-CoA	mutant R88A, pH 7.5, 25°C	Pseudomonas sp.
0.005	-	4-hydroxybenzoyl-CoA	mutant R126L, pH 7.5, 25°C	Pseudomonas sp.
0.0052	-	4-hydroxybenzoyl-CoA	mutant R89L, pH 7.5, 25°C	Pseudomonas sp.
0.006	-	4-hydroxybenzoyl-CoA	wild-type enzyme, pH 7.5, 25°C	Pseudomonas sp.
0.0073	-	4-hydroxybenzoyl-CoA	mutant R128A, pH 7.5, 25°C	Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-Hydroxybenzoyl-CoA + H2O	Pseudomonas sp.	-	4-Hydroxybenzoate + CoA	-	?
4-Hydroxybenzoyl-CoA + H2O	Pseudomonas sp. CBS3	-	4-Hydroxybenzoate + CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	-	-
Pseudomonas sp. CBS3	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
4-Hydroxybenzoyl-CoA + H2O = 4-hydroxybenzoate + CoA	catalytic sites of wild-type and mutant enzymes, substrate binding modifications, reaction kinetics	Pseudomonas sp.	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-Hydroxybenzoyl-CoA + H2O	-	Pseudomonas sp.	4-Hydroxybenzoate + CoA	-	?
4-Hydroxybenzoyl-CoA + H2O	-	Pseudomonas sp. CBS3	4-Hydroxybenzoate + CoA	-	?

Synonyms

Synonyms	Comment	Organism
4-HBA-CoA thioesterase	-	Pseudomonas sp.
4-hydroxybenzoyl coenzyme A hydrolase	-	Pseudomonas sp.
4-hydroxybenzoyl coenzyme A thioesterase	-	Pseudomonas sp.
Hydrolase, 4-hydroxybenzoyl coenzyme A	-	Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00055	-	4-hydroxybenzoyl-CoA	mutant D17N, pH 7.5, 25°C	Pseudomonas sp.
0.00084	-	4-hydroxybenzoyl-CoA	mutant D17S, pH 7.5, 25°C	Pseudomonas sp.
0.11	-	4-hydroxybenzoyl-CoA	mutant R126L, pH 7.5, 25°C	Pseudomonas sp.
0.13	-	4-hydroxybenzoyl-CoA	mutant R88A, pH 7.5, 25°C	Pseudomonas sp.
0.14	-	4-hydroxybenzoyl-CoA	mutant K90A, pH 7.5, 25°C	Pseudomonas sp.
0.15	-	4-hydroxybenzoyl-CoA	mutant R128A, pH 7.5, 25°C	Pseudomonas sp.
0.18	-	4-hydroxybenzoyl-CoA	wild-type enzyme and mutant R89L, pH 7.5, 25°C	Pseudomonas sp.
0.48	-	4-hydroxybenzoyl-CoA	mutant D17E, pH 7.5, 25°C	Pseudomonas sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0011	-	4-hydroxyphenacyl-CoA	mutant R126L, pH 7.5, 25°C	Pseudomonas sp.
0.0012	-	4-hydroxyphenacyl-CoA	mutant R88A, pH 7.5, 25°C	Pseudomonas sp.
0.0014	-	4-hydroxyphenacyl-CoA	wild-type enzyme, pH 7.5, 25°C	Pseudomonas sp.
0.0018	-	4-hydroxyphenacyl-CoA	mutant D17E, pH 7.5, 25°C	Pseudomonas sp.
0.0021	-	4-hydroxyphenacyl-CoA	mutant R128A, pH 7.5, 25°C	Pseudomonas sp.
0.004	-	4-hydroxyphenacyl-CoA	mutant K90A, pH 7.5, 25°C	Pseudomonas sp.

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Release 2023.1 (January 2023)