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Literature summary for 3.1.2.1 extracted from
- Enzymatic and transcriptional regulation of the cytoplasmic acetyl-CoA hydrolase ACOT12 (2013), J. Lipid Res., 54, 2049-2059.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1,2-dimyristoylphosphatidylglycerol | activates mutant form lacking START domain | Mus musculus |  |
| 1-oleoyl-2-lysophosphatidylglycerol | activates mutant form lacking START domain | Mus musculus | |
| Dopa | activates mutant form lacking START domain | Mus musculus | |
| phosphatidic acid | activates mutant form lacking START domain | Mus musculus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | mutant form of ACOT12 lacking the START domain is not inhibited by lipids. Mutant forms both dimers and tetramers whereas wild-type forms only tetramers after addition of ATP | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,2-dimyristoyl phosphatidylglycerol | - |
Mus musculus | |
| 1-oleoyl-2-lysophosphatidic acid | - |
Mus musculus | |
| Dopa | - |
Mus musculus | |
| phosphatidic acid | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
acetoacetyl-CoA | pH 8, 37°C, Vmax: 30 micromol/min/mg, wild-type | Mus musculus | |
| 0.36 | - |
acetyl-CoA | pH 8, 37°C, Vmax: 125 micromol/min/mg, wild-type | Mus musculus | |
| 0.36 | - |
acetyl-CoA | pH 8, 37°C, Vmax: 23.4 micromol/min/mg, mutant form lacking START domain | Mus musculus | |
| 0.41 | - |
butyryl-CoA | pH 8, 37°C, Vmax: 44 micromol/min/mg, wild-type | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
| cytosol | - |
Mus musculus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
4 * 77000 kDa, 45% of wild-type form active tetramers. 55.7% of mutant form lacking START domain form a mixture of active tetramers (31%) and dimers 23.9% | Mus musculus |
| 88000 | - |
gel filtration, mutant form lacking START domain, pre-incubation with ATP, dimer | Mus musculus |
| 182000 | - |
gel filtration, mutant form lacking START domain, pre-incubation with ATP, tetramer | Mus musculus |
| 322000 | - |
gel filtration, wild-type, pre-incubation with ATP, tetramer | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q9DBK0 | - |
- |
| Rattus norvegicus | Q99NB7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
| liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15 | - |
pH 8, 37°C, mutant form lacking START domain | Mus musculus |
| 74.4 | - |
pH 8, 37°C, wild-type | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetoacetyl-CoA + H2O | - |
Mus musculus | CoA + acetoacetate | - |
? | |
| acetyl-CoA + H2O | - |
Mus musculus | CoA + acetate | - |
? | |
| butyryl-CoA + H2O | - |
Mus musculus | CoA + butanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 55.7% of mutant form lacking START domain form a mixture of active tetramers (31%) and dimers (23.9%) | Mus musculus |
| tetramer | 4 * 77000 kDa, 45% of wild-type form active tetramers. 55.7% of mutant form lacking START domain form a mixture of active tetramers (31%) and dimers 23.9% | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetyl-CoA hydrolase | - |
Rattus norvegicus |
| acetyl-CoA hydrolase | - |
Mus musculus |
| Acot12 | - |
Rattus norvegicus |
| Acot12 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.99 | - |
acetyl-CoA | pH 8, 37°C, mutant form lacking START domain | Mus musculus | |
| 7.26 | - |
acetyl-CoA | pH 8, 37°C, wild-type | Mus musculus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Mus musculus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Rattus norvegicus | ACOT12 mRNA and protein levels in rat primary hepatocytes decrease following treatment with insulin | down |
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