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Literature summary for 3.1.16.1 extracted from

  • Bernardi, A.; Bernardi, G.
    Studies on acid hydrolases. IV. Isolation and characterization of spleen exonuclease (1968), Biochim. Biophys. Acta, 155, 360-370.
    View publication on PubMed
Search for more literature for 3.1.16.1

Application

Application Comment Organism
analysis end group and sequential analysis of polynucleotides Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Sus scrofa 5739
-

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
no contamination by other phosphohydrolase activities Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phospho-2'-deoxyribooligonucleotide + H2O average size 10-12, smallest 3 Sus scrofa 2'-deoxyribonucleoside 3'-phosphate
-
 ?
4-nitrophenyl phosphate
-
 Sus scrofa 4-nitrophenol + phosphate
-
 ?
5'-hydroxy oligonucleotides + H2O dependency on type of nucleobase Sus scrofa nucleoside 3'-oligonucleotides + nucleoside 3'-phosphates
-
 ?
bis-(4-nitrophenyl)phosphate + H2O
-
 Sus scrofa 4-nitrophenyl phosphate + 4-nitrophenol
-
 ?
DNA + H2O alkali-denaturated DNA, 25 times lower degradation rate with native DNA Sus scrofa deoxyribonucleoside 3'-phosphates
-
 ?
polyadenylic acid + H2O
-
 Sus scrofa 5'-hydroxy-adenine oligonucleotides + 3'AMP
-
 ?
polyinosinic acid
-
 Sus scrofa 3'-IMP
-
 ?
polyuridylic acid + H2O
-
 Sus scrofa 3'-UMP
-
 ?
RNA core + H2O RNA core are water undializable 3'-phosphoribooligonucleotides obtained by exhaustive digestion of RNA with pancreatic ribonuclease Sus scrofa nucleoside 3'-phosphates
-
 ?
thymidine 3'-(4-nitrophenyl)phosphate + H2O very slow hydrolysis Sus scrofa thymidine 3'-phosphate + 4-nitrophenol
-
 ?
tRNA + H2O from Escherichia coli Sus scrofa tRNA fragments + nucleoside 3'-phosphates
-
 ?

Synonyms

Synonyms Comment Organism
phosphodiesterase II
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 80 range of thermal inactivation, 50% of activity at 56°C Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 with 0.1 M acetate or succinate buffer Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
4 7 poor effect of 0.02 M Mg2+ on pH activity curve at 37°C Sus scrofa