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Literature summary for 3.1.15.1 extracted from
- Capillary monolithic bioreactor of immobilized snake venom phosphodiesterase for mass spectrometry based oligodeoxynucleotide sequencing (2012), Anal. Chem., 84, 1157-1164.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme reaction is induced by a ubiquitous pollutant acrolein | Crotalus atrox |
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | the enzyme is useful for production of the mass ladders of oligodeoxynucleotides with a single nucleotide difference, which can be produced, allowing for MS-based sequencing, construction of a capillary monolithic bioreactor constructed for the immobilized enzyme and handling (sub)microliter DNA samples | Crotalus atrox |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | immobilization of the enzyme on the capillary monolith, method, overview. The immobilization of the enzyme facilitates the elimination or repression of the metal adducts of oligodeoxynucleotides during the enzyme reaction | Crotalus atrox |
General Stability
| General Stability | Organism |
|---|---|
| instability of the enzyme in aqueous solution | Crotalus atrox |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Crotalus atrox | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Crotalus atrox | the enzyme has the unique ability as an exonuclease to sequentially cleave one nucleotide from single-stranded DNA, MALDI-TOF mass spectrometric analysis of reaction products | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crotalus atrox | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Crotalus atrox | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl thymidine-5'-phosphate + H2O | - |
Crotalus atrox | 4-nitrophenol + thymidine-5'-phosphate | - |
? | |
| additional information | the enzyme has the unique ability as an exonuclease to sequentially cleave one nucleotide from single-stranded DNA, MALDI-TOF mass spectrometric analysis of reaction products | Crotalus atrox | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| snake venom phosphodiesterase | - |
Crotalus atrox |
| SVP | - |
Crotalus atrox |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Crotalus atrox |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
assay at | Crotalus atrox |
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Release 2023.1 (January 2023)
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