KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of purified native enzyme | Trimeresurus stejnegeri | |
0.091 | - |
ADP | pH 7.4, 37°C | Trimeresurus stejnegeri | |
0.24 | - |
nicotinamide adenine dinucleotide | pH 7.4, 37°C | Trimeresurus stejnegeri | |
0.29 | - |
nicotinamide guanine dinucleotide | pH 7.4, 37°C | Trimeresurus stejnegeri | |
0.36 | - |
ATP | pH 7.4, 37°C | Trimeresurus stejnegeri |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trimeresurus stejnegeri | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | essential for phosphodiesterase activity of the enzyme | Trimeresurus stejnegeri | |
Zn2+ | essential for phosphodiesterase activity of the enzyme | Trimeresurus stejnegeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trimeresurus stejnegeri | the enzyme also exhibits DNase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trimeresurus stejnegeri | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the venom phosphodiesterase TS-PDE is a glycoprotein which contains 2.48% carbohydrate | Trimeresurus stejnegeri |
Purification (Comment) | Organism |
---|---|
native enzyme from venom by ion exchange chromatography and gel filtration to homogeneity | Trimeresurus stejnegeri |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | commercial preparation | Trimeresurus stejnegeri | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.3 | - |
purified native enzyme, substrate nicotinamide guanine dinucleotide, pH 7.4, 37°C | Trimeresurus stejnegeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Trimeresurus stejnegeri | ? | - |
? | |
ATP + H2O | - |
Trimeresurus stejnegeri | ? | - |
? | |
additional information | the enzyme also exhibits DNase activity | Trimeresurus stejnegeri | ? | - |
? | |
additional information | the purified enzyme TS-PDE exhibits exonuclease activity with a broad phosphodiesterase substrate range with the descending order of specificity: nicotinamide guanine dinucleotide, ATP, nicotinamide adenine dinucleotide, ADP | Trimeresurus stejnegeri | ? | - |
? | |
nicotinamide adenine dinucleotide + H2O | - |
Trimeresurus stejnegeri | ? | - |
? | |
nicotinamide guanine dinucleotide + H2O | - |
Trimeresurus stejnegeri | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TS-PDE | - |
Trimeresurus stejnegeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trimeresurus stejnegeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
ADP | pH 7.4, 37°C | Trimeresurus stejnegeri | |
0.82 | - |
nicotinamide adenine dinucleotide | pH 7.4, 37°C | Trimeresurus stejnegeri | |
2.68 | - |
ATP | pH 7.4, 37°C | Trimeresurus stejnegeri | |
3.65 | - |
nicotinamide guanine dinucleotide | pH 7.4, 37°C | Trimeresurus stejnegeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Trimeresurus stejnegeri |
General Information | Comment | Organism |
---|---|---|
physiological function | the phosphodiesterase TS-PDE strongly inhibits ADP-induced platelet aggregation in human plateletrich plasma by hydrolyzing ADP | Trimeresurus stejnegeri |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
ADP | pH 7.4, 37°C | Trimeresurus stejnegeri | |
3.5 | - |
nicotinamide adenine dinucleotide | pH 7.4, 37°C | Trimeresurus stejnegeri | |
7.4 | - |
ATP | pH 7.4, 37°C | Trimeresurus stejnegeri | |
12.6 | - |
nicotinamide guanine dinucleotide | pH 7.4, 37°C | Trimeresurus stejnegeri |