Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.14.1 extracted from

  • Nakashima, A.; Yoshida, M.; Nakayama, K.; Kato-Furuno, A.; Ueno, M.; Ushimaru, T.; Uritani, M.
    Genes for a nuclease and a protease are involved in the drastic decrease in cellular RNA amount in fission yeast cells during nitrogen starvation (2002), J. Biochem., 131, 391-398.
    View publication on PubMed
Search for more literature for 3.1.14.1

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme is induced during nitrogen starvation Schizosaccharomyces pombe
protease isp6+ strongly dependent on, gene isp6+ encodes a protease, which is involved in RNA-degrading enzyme activity, an isp6+-deficient mutant is also devoid of RNA-degrading enzyme activity Schizosaccharomyces pombe

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type enzyme in Schizosaccharomyces pombe Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
additional information construction of a enzyme null-mutant pnu1DELTA, pnu1+ is dispensable for mitochondrial function and not essential for cell growth Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
EDTA recoverage by either Mg2+, Cl-, or CaCl2 Schizosaccharomyces pombe

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Schizosaccharomyces pombe 5739
-
soluble
-
 Schizosaccharomyces pombe
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, SDS-PAGE Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA + H2O Schizosaccharomyces pombe
-
 nucleoside 3'-phosphates
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
 gene pnu1+
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type enzyme from overexpressing cells Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O pUC18 or pSP1 Schizosaccharomyces pombe deoxynucleoside 3'-phosphates
-
 ?
additional information the enzyme is not essential for degradation of 26S and 17S rRNA Schizosaccharomyces pombe ?
-
 ?
RNA + H2O
-
 Schizosaccharomyces pombe nucleoside 3'-phosphates
-
 ?
RNA + H2O total RNA from Schizosaccharomyces pombe Schizosaccharomyces pombe nucleoside 3'-phosphates
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
nuclease 1
-
 Schizosaccharomyces pombe
RNA-degrading enzyme
-
 Schizosaccharomyces pombe

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Schizosaccharomyces pombe